Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,426,403	G→A	G113R (GGG→AGG)	yfcG →	predicted glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,426,403	0	G	A	100.0%	108.9 / NA	38	G113R (GGG→AGG)	yfcG	predicted glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/8);  total (30/8)

AGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGG  >  W3110S.gb/2426344‑2426469                                                            |                                                                   aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat                                                         >  1:3816588/1‑71 (MQ=255) aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat                                                         >  1:4377599/1‑71 (MQ=255) aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat                                                         <  1:197728/71‑1 (MQ=255) aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat                                                         >  1:546548/1‑71 (MQ=255)   cgcgcCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAAtcatca                                                       <  1:2912821/71‑1 (MQ=255)       ccgccACATTACAGTGGTTATTGTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCAtttt                                                   <  1:383138/71‑1 (MQ=255)          ccACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAAt                                                <  1:1946874/71‑1 (MQ=255)                 aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCa                                              >  1:738943/1‑66 (MQ=255)                 aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCa                                              >  1:4659866/1‑66 (MQ=255)                 aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg                                         >  1:1910756/1‑71 (MQ=255)                 aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg                                         >  1:4650506/1‑71 (MQ=255)                 aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg                                         >  1:1188799/1‑71 (MQ=255)                 aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg                                         >  1:4543550/1‑71 (MQ=255)                 aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg                                         >  1:3841156/1‑71 (MQ=255)                 aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg                                         >  1:114474/1‑71 (MQ=255)                    gTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGccc                                      >  1:1172174/1‑71 (MQ=255)                          aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCaaa                                >  1:2712121/1‑71 (MQ=255)                          aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCaaa                                >  1:2997424/1‑71 (MQ=255)                          aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCaaa                                >  1:4610049/1‑71 (MQ=255)                                gCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCa                             <  1:4508239/68‑1 (MQ=255)                                     tAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc                     >  1:1521419/1‑71 (MQ=255)                                      aGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACg                    >  1:1099393/1‑71 (MQ=255)                                      aGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACg                    >  1:3726228/1‑71 (MQ=255)                                      aGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACg                    >  1:1866194/1‑71 (MQ=255)                                      aGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACg                    >  1:1066417/1‑71 (MQ=255)                                       ggCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGc                   <  1:4574421/71‑1 (MQ=255)                                         cGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc                                           >  1:4094466/1‑45 (MQ=255)                                            tcTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCt                  <  1:3467883/66‑1 (MQ=255)                                             cTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGcc                                       >  1:1675466/1‑45 (MQ=255)                                             cTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTAtt               >  1:4211200/1‑69 (MQ=255)                                                gggCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt                        >  1:4218685/1‑57 (MQ=255)                                                  gCCGATGCTTAGGCAAAATCATCATTTTAATCACGCATCCCCCCAAACCATTCCTTACGCTATTGAACGtt        <  1:3674273/71‑1 (MQ=255)                                                        gCTTAGGCAAAATCATCATTTTAATCACGCCGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg  >  1:4008348/1‑71 (MQ=255)                                                        gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg  >  1:3867118/1‑71 (MQ=255)                                                        gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg  >  1:3688499/1‑71 (MQ=255)                                                        gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg  >  1:988405/1‑71 (MQ=255)                                                        gCTTAGGCAAAATCATCATTTTAATAACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg  >  1:2672125/1‑71 (MQ=255)                                                         cTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTa       >  1:3715255/1‑65 (MQ=255)                                                            |                                                                   AGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGG  >  W3110S.gb/2426344‑2426469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑