Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,430,751	G→T	F204L (TTC→TTA)	hisQ ←	histidine/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,430,751	0	G	T	100.0%	147.8 / NA	50	F204L (TTC→TTA)	hisQ	histidine/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/31);  total (19/31)

TCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCC  >  W3110S.gb/2430683‑2430813                                                                     |                                                               tCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTaa                                                              <  1:3908817/71‑1 (MQ=255)     cccTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAAcc                                                          <  1:1642805/71‑1 (MQ=255)      ccTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAAcc                                                          <  1:4193979/70‑1 (MQ=255)      ccTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCa                                                         <  1:4283226/71‑1 (MQ=255)          ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAgga                                                      >  1:4351366/1‑69 (MQ=255)          ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAgg                                                       >  1:773221/1‑69 (MQ=255)          ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGt                                                      >  1:4521913/1‑70 (MQ=255)          ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGt                                                      >  1:2224928/1‑70 (MQ=255)            cacaCCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTaaaa                                                            >  1:2386973/1‑62 (MQ=255)            cacaCCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTaa                                                    <  1:4182675/70‑1 (MQ=255)             acacCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAAt                                                  <  1:4508551/71‑1 (MQ=255)             acacCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAAt                                                  <  1:468438/71‑1 (MQ=255)             acacCCACGGAGTAGCCGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAAt                                                  <  1:650409/71‑1 (MQ=255)              cacCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAAt                                                  >  1:615340/1‑70 (MQ=255)                cccACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCAc                                               <  1:4027793/71‑1 (MQ=255)                  cACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGc                                             >  1:3477494/1‑71 (MQ=255)                    cGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCca                                           <  1:1055695/71‑1 (MQ=255)                      gAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCcaca                                         <  1:318080/71‑1 (MQ=255)                         tAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc                                      <  1:2933967/71‑1 (MQ=255)                         tAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc                                      <  1:1643494/71‑1 (MQ=255)                           gcggcgCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGa                                    >  1:156103/1‑71 (MQ=255)                                 cTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGa                              <  1:451027/71‑1 (MQ=255)                                 cTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGa                              <  1:4672769/71‑1 (MQ=255)                                      ggAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGaaa                            >  1:2279361/1‑68 (MQ=255)                                      ggAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAg                         <  1:3717553/71‑1 (MQ=255)                                        aaCAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTaaa                                                   >  1:1471100/1‑43 (MQ=255)                                            gcagcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGt                   <  1:2031834/71‑1 (MQ=255)                                             cagcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGtt                  >  1:935181/1‑71 (MQ=255)                                             cagcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGtt                  >  1:1117919/1‑71 (MQ=255)                                               gcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc                                      <  1:2943500/49‑1 (MQ=255)                                               gcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc                                      <  1:2979584/49‑1 (MQ=255)                                                    ccATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGtac                 >  1:1667257/1‑63 (MQ=255)                                                    ccATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAgg            >  1:2765929/1‑70 (MQ=255)                                                    ccATTGGAAACAGTGGTTAAAAACAGGTAAAACACGCCACAGACGAAGGCg                               >  1:2843966/1‑51 (MQ=255)                                                      aTTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTAc         <  1:3942230/71‑1 (MQ=255)                                                      aTTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTAc         <  1:1464484/71‑1 (MQ=255)                                                       ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc                                      <  1:346328/41‑1 (MQ=255)                                                       ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt        <  1:3425314/71‑1 (MQ=255)                                                       ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt        <  1:3958133/71‑1 (MQ=255)                                                       ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt        <  1:517063/71‑1 (MQ=255)                                                       ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt        <  1:52487/71‑1 (MQ=255)                                                       ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt        <  1:4079324/71‑1 (MQ=255)                                                         ggAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGaaa                            >  1:893052/1‑49 (MQ=255)                                                         ggAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACttt      >  1:437754/1‑71 (MQ=255)                                                          gAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACttt      <  1:2836528/70‑1 (MQ=255)                                                           aaaCAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTtg    <  1:484485/71‑1 (MQ=255)                                                           aaaCAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTtg    <  1:2222802/71‑1 (MQ=255)                                                              cAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTccc               <  1:371583/57‑1 (MQ=255)                                                              cAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTcc                >  1:4100943/1‑56 (MQ=255)                                                              cAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTtgcc  >  1:4066675/1‑70 (MQ=255)                                                                     |                                                               TCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCC  >  W3110S.gb/2430683‑2430813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑