| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,464,504 | C→G | E34D (GAG→GAC) | yfcX ← | fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,464,504 | 0 | C | G | 98.0% | 160.0 / ‑4.2 | 49 | E34D (GAG→GAC) | yfcX | fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (25/23); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
AACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA > W3110S.gb/2464436‑2464568 | aaCACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTc > 1:3948926/1‑71 (MQ=255) acacCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTcc < 1:4159227/71‑1 (MQ=255) caccacGCCTCGCAACTCTTTGTTTTCATGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCg > 1:3944331/1‑71 (MQ=255) acGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTc > 1:1810679/1‑65 (MQ=255) gCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCtt > 1:2033739/1‑69 (MQ=255) aCTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTc < 1:4330347/71‑1 (MQ=255) aCTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTc < 1:2459663/71‑1 (MQ=255) ctTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCAtt > 1:437761/1‑71 (MQ=255) ctTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCAtt > 1:1597136/1‑71 (MQ=255) ctTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCAtt > 1:2006949/1‑71 (MQ=255) ctTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCAtt > 1:3294970/1‑71 (MQ=255) ctTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCAtt > 1:3094970/1‑71 (MQ=255) ctTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCAtt > 1:3235692/1‑71 (MQ=255) tGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCAtt < 1:2545893/68‑1 (MQ=255) tGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTtc > 1:825243/1‑71 (MQ=255) ttttCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTtct < 1:3383803/70‑1 (MQ=255) cGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCgg < 1:1295827/71‑1 (MQ=255) cGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCgg < 1:2153974/71‑1 (MQ=255) gTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCgg < 1:137379/67‑1 (MQ=255) ttGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTAtt < 1:1974221/51‑1 (MQ=255) ttGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTAtt < 1:3737970/51‑1 (MQ=255) tGCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc > 1:575075/1‑71 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCTCCTTCAGGGTATTCATTTTCTCACCCGGTa > 1:4282444/1‑66 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGtg < 1:3794330/70‑2 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc > 1:2792456/1‑70 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc < 1:606966/70‑1 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc > 1:4271754/1‑70 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc > 1:3163246/1‑70 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc > 1:263722/1‑70 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc < 1:2602647/70‑1 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc < 1:2515189/70‑1 (MQ=255) gCTTAATAATGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc < 1:3283110/70‑1 (MQ=255) taataaTGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCAccc > 1:3577428/1‑59 (MQ=255) atGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCAcc > 1:3684020/1‑53 (MQ=255) atGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGa > 1:682846/1‑70 (MQ=255) atGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGa > 1:2280762/1‑70 (MQ=255) atGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGAt < 1:4287938/71‑1 (MQ=255) atGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGAt < 1:2987846/71‑1 (MQ=255) atGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGAt < 1:3074430/71‑1 (MQ=255) atGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGAt < 1:425007/71‑1 (MQ=255) atGGCGCGCACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGAt < 1:2181805/71‑1 (MQ=255) gcgcgcACCTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAAc > 1:3055174/1‑71 (MQ=255) ccTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGAt < 1:3808383/61‑1 (MQ=255) ccTGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtg < 1:4537787/71‑1 (MQ=255) tGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCa > 1:1383807/1‑39 (MQ=255) tGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCa > 1:4242114/1‑39 (MQ=255) tGCGAGGCAAAGTCCGCCTTCAGGGTATTCATTTTCTCa > 1:3798795/1‑39 (MQ=255) gCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTa > 1:3803346/1‑40 (MQ=255) gCAAAGTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCa < 1:538403/70‑1 (MQ=255) | AACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA > W3110S.gb/2464436‑2464568 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |