Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,512,230	A→G	W371R (TGG→CGG)	ypdG ←	predicted enzyme IIC component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,512,230	0	A	G	100.0%	120.4 / NA	36	W371R (TGG→CGG)	ypdG	predicted enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/13);  total (23/13)

TTATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAGCG  >  W3110S.gb/2512164‑2512297                                                                   |                                                                    ttATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGa                                                                   <  1:1960568/69‑1 (MQ=255)   aTGACTGCCCCCAGCGCGATCCTCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGc                                                               >  1:1234454/1‑71 (MQ=255)   aTGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAg                                                                >  1:521954/1‑70 (MQ=255)    tGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCg                                                              <  1:550750/71‑1 (MQ=255)       cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGTCCATGCCCGGATAGCGGAc                                                           <  1:954324/71‑1 (MQ=255)         gCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACTAGCGGCCATGCCCGGATAGCGGACTc                                                         >  1:214424/1‑71 (MQ=255)                 cgcgATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAAcc                                                 <  1:1486626/71‑1 (MQ=255)                  gcgATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCa                                                >  1:2271472/1‑71 (MQ=255)                     aTCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTg                                             <  1:3452973/71‑1 (MQ=255)                      tCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTg                                             <  1:4447456/70‑1 (MQ=255)                                ataGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGcc                                    >  1:4492414/1‑69 (MQ=255)                                 taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag                                 >  1:1888438/1‑71 (MQ=255)                                 taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag                                 >  1:2766707/1‑71 (MQ=255)                                 taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag                                 >  1:1765375/1‑71 (MQ=255)                                 taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag                                 >  1:530116/1‑71 (MQ=255)                                 taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag                                 >  1:1523125/1‑71 (MQ=255)                                 taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag                                 >  1:4299022/1‑71 (MQ=255)                                 taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag                                 >  1:4270261/1‑71 (MQ=255)                                 taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag                                 >  1:4217587/1‑71 (MQ=255)                                    aCGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccagcca                              <  1:4182725/71‑1 (MQ=255)                                              tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcggcg                    >  1:16880/1‑71 (MQ=255)                                               aGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGcggcgg                   >  1:457148/1‑71 (MQ=255)                                                gTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGcggcgg                   <  1:3162928/70‑1 (MQ=255)                                                gTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGcggcgg                   <  1:3996682/70‑1 (MQ=255)                                                         ggCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc         <  1:976808/71‑1 (MQ=255)                                                          gCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc         <  1:406681/70‑1 (MQ=255)                                                              tGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc         <  1:3917318/66‑1 (MQ=255)                                                              tGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc         <  1:371586/66‑1 (MQ=255)                                                               gCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAgc   >  1:4397631/1‑71 (MQ=255)                                                                cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCGGACAGCGGCGGTTGAGCCGACAATAgc   >  1:1338771/1‑70 (MQ=255)                                                                cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGAc                           >  1:3789353/1‑46 (MQ=255)                                                                cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACaa       >  1:3944892/1‑66 (MQ=255)                                                                cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACaa       >  1:3646258/1‑66 (MQ=255)                                                                cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAgcg  >  1:3286219/1‑71 (MQ=255)                                                                cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAgcg  >  1:3021993/1‑71 (MQ=255)                                                                cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAgcg  >  1:1767310/1‑71 (MQ=255)                                                                   |                                                                    TTATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAGCG  >  W3110S.gb/2512164‑2512297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑