Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,585,491	T→C	L369L (TTG→CTG)	narQ →	sensory histidine kinase in two‑component regulatory system with NarP (NarL)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,585,491	0	T	C	100.0%	222.7 / NA	65	L369L (TTG→CTG)	narQ	sensory histidine kinase in two‑component regulatory system with NarP (NarL)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/29);  total (36/29)

TTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAT  >  W3110S.gb/2585423‑2585558                                                                     |                                                                    ttAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTg                                                                   >  1:3387289/1‑71 (MQ=255)  tAATCAGGTGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTg                                                                   >  1:3724631/1‑70 (MQ=255)  tAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTg                                                                   >  1:1970722/1‑70 (MQ=255)                 gCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTc                                                   <  1:3470479/71‑1 (MQ=255)                    ttttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAgg                                                <  1:12877/71‑1 (MQ=255)                      ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTg                                                                   >  1:1241982/1‑50 (MQ=255)                      ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGc                                                                  >  1:352520/1‑51 (MQ=255)                      ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGc                                                     >  1:4092146/1‑64 (MQ=255)                      ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGt                                               >  1:4387228/1‑70 (MQ=255)                      ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGt                                               >  1:589192/1‑70 (MQ=255)                      ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTa                                              >  1:1817820/1‑71 (MQ=255)                      ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTa                                              >  1:402344/1‑71 (MQ=255)                      ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTa                                              >  1:382346/1‑71 (MQ=255)                      ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTa                                              >  1:1980648/1‑71 (MQ=255)                            aaTTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTct                                        <  1:2045642/71‑1 (MQ=255)                             attattGTTGATGGAAGAACGGGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTc                                                   <  1:2346396/59‑1 (MQ=38)                               tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCa                                                                 >  1:878976/1‑43 (MQ=255)                               tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAg                                                 >  1:1729243/1‑59 (MQ=255)                               tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttac                                     >  1:4307730/1‑71 (MQ=255)                               tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttac                                     >  1:576645/1‑71 (MQ=255)                               tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttac                                     >  1:1337492/1‑71 (MQ=255)                               tattGTTGATGGAAGAACGTGCGACAATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttac                                     >  1:2897314/1‑71 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGAc                                                             >  1:768009/1‑46 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCt                                                        >  1:2396631/1‑51 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGTCTCAGGTAc                                             >  1:1888870/1‑62 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTAc                                             >  1:2407388/1‑62 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACt                                            >  1:982808/1‑63 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact                                    >  1:1360360/1‑71 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact                                    >  1:3176244/1‑71 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact                                    >  1:735603/1‑71 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact                                    >  1:3447390/1‑71 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact                                    >  1:3496077/1‑71 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact                                    >  1:2436783/1‑71 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact                                    >  1:4630373/1‑71 (MQ=255)                                attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTctta                                      >  1:66488/1‑69 (MQ=255)                                 ttgttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact                                    <  1:4214053/70‑1 (MQ=255)                                   gttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttactta                                  <  1:240889/70‑1 (MQ=255)                                   gttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttactta                                  <  1:4382920/70‑1 (MQ=255)                                   gttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCttacttac                                 >  1:1469151/1‑71 (MQ=255)                                    ttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACg                                <  1:58788/71‑1 (MQ=255)                                        tGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACttt                                          <  1:2821744/57‑1 (MQ=255)                                        tGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATc                            <  1:1035433/71‑1 (MQ=255)                                        tGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATc                            <  1:4452140/71‑1 (MQ=255)                                          gaagaaCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATcc                           <  1:4550828/70‑1 (MQ=255)                                            agaaCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTctt                                       <  1:4428722/56‑1 (MQ=255)                                               aCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTg                      <  1:1729147/70‑1 (MQ=255)                                               aCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGa                     <  1:1038273/71‑1 (MQ=255)                                               aCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGa                     <  1:3707499/71‑1 (MQ=255)                                                cGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGAc                    >  1:977045/1‑71 (MQ=255)                                                cGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGAc                    <  1:1587476/71‑1 (MQ=255)                                                  tGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGt                  <  1:952896/71‑1 (MQ=255)                                                    cGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGtt                 <  1:1875367/70‑1 (MQ=255)                                                    cGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGtt                 <  1:2532533/70‑1 (MQ=255)                                                    cGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTa                >  1:3063786/1‑71 (MQ=255)                                                       ccATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTg             <  1:2968778/71‑1 (MQ=255)                                                        cATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGtt                 >  1:3459628/1‑66 (MQ=255)                                                            gCCCGCGTACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCg        <  1:3689776/71‑1 (MQ=255)                                                            gCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGc         <  1:3345577/70‑1 (MQ=255)                                                            gCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCg        >  1:1496661/1‑71 (MQ=255)                                                              ccGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGt       <  1:2569319/70‑1 (MQ=255)                                                               cgcgAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTc     <  1:4322682/71‑1 (MQ=255)                                                                  gAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAt  <  1:1983970/71‑1 (MQ=255)                                                                  gAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAt  <  1:4193090/71‑1 (MQ=255)                                                                  gAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAt  <  1:1828424/71‑1 (MQ=255)                                                                  gAACTGCACGACTCGCTGGCTCAAGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAt  <  1:4085778/71‑1 (MQ=255)                                                                     |                                                                    TTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAT  >  W3110S.gb/2585423‑2585558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑