Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	470,819	A→G	E320E (GAA→GAG)	mdlB →	fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	470,819	0	A	G	100.0%	329.4 / NA	94	E320E (GAA→GAG)	mdlB	fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/54);  total (40/54)

TTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAG  >  W3110S.gb/470751‑470886                                                                     |                                                                    ttAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt                                                                   <  1:4095127/71‑1 (MQ=255) ttAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt                                                                   <  1:2834416/71‑1 (MQ=255) ttAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt                                                                   <  1:3127115/71‑1 (MQ=255) ttAATCGAACTGACCACGCAACACGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt                                                                   <  1:3732400/71‑1 (MQ=255)  tAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt                                                                   >  1:3589089/1‑70 (MQ=255)  tAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt                                                                   >  1:3690433/1‑70 (MQ=255)  tAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt                                                                   >  1:181420/1‑70 (MQ=255)   aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTg                                                                  <  1:285535/70‑1 (MQ=255)   aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa                                                                 >  1:1156954/1‑71 (MQ=255)   aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa                                                                 >  1:758789/1‑71 (MQ=255)   aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa                                                                 >  1:2860707/1‑71 (MQ=255)   aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa                                                                 >  1:1457921/1‑71 (MQ=255)   aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa                                                                 >  1:617273/1‑71 (MQ=255)   aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa                                                                 >  1:4582389/1‑71 (MQ=255)   aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa                                                                 >  1:1096771/1‑71 (MQ=255)    aTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGAt                                                                >  1:4613702/1‑71 (MQ=255)     tCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATg                                                               <  1:1095890/71‑1 (MQ=255)       gAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATgga                                                             >  1:1010922/1‑71 (MQ=255)          cTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGa                                                                 >  1:4071046/1‑64 (MQ=255)          cTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATggacgg                                                          <  1:1884713/71‑1 (MQ=255)          cTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATggacg                                                           >  1:750901/1‑70 (MQ=255)             aCCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGATGGTGAGCGCGTGTTTGAGCTGATGGACGGAcc                                                       >  1:2324765/1‑71 (MQ=255)             aCCACGAAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGAcc                                                       >  1:600401/1‑71 (MQ=255)                 cGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCgcgc                                                   >  1:1436701/1‑71 (MQ=255)                  gCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGcc                                                  <  1:3977539/71‑1 (MQ=255)                   cAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCgcgc                                                   >  1:3209318/1‑69 (MQ=255)                     aCAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGc                                               <  1:1032232/71‑1 (MQ=255)                     aCAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGc                                               <  1:3472695/71‑1 (MQ=255)                      cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATggacgg                                                          <  1:770422/59‑1 (MQ=255)                      cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGc                                               >  1:2490440/1‑70 (MQ=255)                      cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGc                                               >  1:1336617/1‑70 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGGGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             >  1:350274/1‑71 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:689690/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:979996/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:363189/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:626449/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:3823550/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:439534/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:1282623/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:1581152/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:1466697/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:2097733/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:1875746/71‑1 (MQ=255)                       aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             <  1:221625/71‑1 (MQ=255)                        ggCGATGCTGCAACAGGCTGTTGTTGCTGGTGCGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa                                             >  1:589499/1‑70 (MQ=255)                            aTGCTGCAAAAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt                                                                   <  1:3681926/44‑1 (MQ=38)                             tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc                                       <  1:1357336/71‑1 (MQ=255)                             tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc                                       <  1:1153065/71‑1 (MQ=255)                             tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc                                       <  1:4056170/71‑1 (MQ=255)                             tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc                                       <  1:1467650/71‑1 (MQ=255)                             tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc                                       <  1:4276549/71‑1 (MQ=255)                             tgctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc                                       <  1:3766995/71‑1 (MQ=255)                              gctgcAACAGACTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGc                                       >  1:1811000/1‑70 (MQ=255)                                  cAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatg                                   <  1:3332162/70‑1 (MQ=255)                                  cAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAat                                    <  1:3534497/69‑1 (MQ=255)                                    aCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg                                <  1:1488569/71‑1 (MQ=255)                                    aCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg                                <  1:1467125/71‑1 (MQ=255)                                    aCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgat                                 >  1:673800/1‑70 (MQ=255)                                    aCAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgat                                 >  1:3930806/1‑70 (MQ=255)                                    aCAGGCTGTTGCTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg                                <  1:4219831/71‑1 (MQ=255)                                        gCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCg                            >  1:606698/1‑71 (MQ=255)                                          tgttgtTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGcc                          >  1:2359134/1‑71 (MQ=255)                                            ttgttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg                                <  1:384684/63‑1 (MQ=255)                                              gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt                       <  1:3659337/70‑1 (MQ=255)                                              gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt                       >  1:1910874/1‑70 (MQ=255)                                              gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt                       >  1:3886615/1‑70 (MQ=255)                                              gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt                       <  1:737107/70‑1 (MQ=255)                                              gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt                       >  1:1856991/1‑70 (MQ=255)                                              gttgCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGt                       <  1:1600381/70‑1 (MQ=255)                                                  cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag                  <  1:1995537/71‑1 (MQ=255)                                                  cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag                  <  1:2172757/71‑1 (MQ=255)                                                  cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag                  <  1:1343470/71‑1 (MQ=255)                                                  cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag                  <  1:3388875/71‑1 (MQ=255)                                                  cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag                  <  1:1309671/71‑1 (MQ=255)                                                  cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag                  <  1:232526/71‑1 (MQ=255)                                                  cTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACa                   <  1:2780253/70‑1 (MQ=255)                                                     gTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGt               >  1:4288967/1‑71 (MQ=255)                                                       gAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCATTACAGAGTgg             >  1:2319872/1‑71 (MQ=255)                                                        aGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATgatgat                              >  1:2846923/1‑53 (MQ=255)                                                        aGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTgg             >  1:3676376/1‑70 (MQ=255)                                                        aGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTgg             <  1:76212/70‑1 (MQ=255)                                                         gcgcGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGACCGTTACag                  <  1:3955358/64‑1 (MQ=255)                                                            cgTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag                  <  1:2447175/61‑1 (MQ=255)                                                               gtTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAattatg                                <  1:3607387/44‑1 (MQ=38)                                                               gtTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg                                <  1:3308080/44‑1 (MQ=255)                                                                tttGAGCTGATGGACTGACCGCGCCAGCAATATGGCAat                                    <  1:56765/39‑1 (MQ=37)                                                                tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAat                                    <  1:4388207/39‑1 (MQ=255)                                                                tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAat                                    <  1:3156033/39‑1 (MQ=255)                                                                tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATgatgat                              >  1:4485042/1‑45 (MQ=255)                                                                tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCg     >  1:2183352/1‑70 (MQ=255)                                                                tttGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCg     >  1:1134604/1‑70 (MQ=255)                                                                  tGAGCTGATGGACGGACCGCGCCAGCAATGTGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAg  >  1:4208378/1‑71 (MQ=255)                                                                  tGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAg  >  1:4543255/1‑71 (MQ=255)                                                                  tGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAg  >  1:3249037/1‑71 (MQ=255)                                                                     |                                                                    TTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAG  >  W3110S.gb/470751‑470886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑