Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,793,641	A→C	I245L (ATT→CTT)	gabP →	gamma‑aminobutyrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,793,641	0	A	C	100.0%	240.7 / NA	74	I245L (ATT→CTT)	gabP	gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/53);  total (21/53)

GCCGCCGCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTATTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGAATATGCCCG  >  W3110S.gb/2793575‑2793704                                                                   |                                                                gccgccGCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCtt                                                               >  1:1318751/1‑69 (MQ=255) gccgccGCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTc                                                             >  1:235993/1‑71 (MQ=255) gccgccGCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTc                                                             >  1:2069849/1‑71 (MQ=255)  ccgccgCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTc                                                             <  1:3918295/70‑1 (MQ=255)  ccgccgCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTc                                                             <  1:2180580/70‑1 (MQ=255)  ccgccgCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTc                                                             <  1:3173095/70‑1 (MQ=255)  ccgccgCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCt                                                            <  1:4685780/71‑1 (MQ=255)   cgccgcGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTa                                                           <  1:794256/71‑1 (MQ=255)   cgccgcGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTa                                                           <  1:1674462/71‑1 (MQ=255)        cgGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTAtcttc                                                      <  1:266185/71‑1 (MQ=255)               cGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTAtttt                                                >  1:2781409/1‑69 (MQ=255)               cGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTa                                                >  1:4662953/1‑70 (MQ=255)               cGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTa                                                <  1:2582013/70‑1 (MQ=255)               cGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTa                                                <  1:773159/70‑1 (MQ=255)               cGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTa                                                >  1:326199/1‑70 (MQ=255)               cGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTAt                                               >  1:3093910/1‑71 (MQ=255)                   acGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTa                                                           >  1:3453762/1‑55 (MQ=255)                              aCATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCtt                                                               >  1:2385067/1‑40 (MQ=255)                               cATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATTTTCTATTTTTgtcg                                >  1:2332549/1‑70 (MQ=255)                               cATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTgtcg                                >  1:4238110/1‑70 (MQ=255)                               cATATTGTCCGCGCCAACAACTCGGTTATCTGGCGTCTTTc                                                             >  1:2161851/1‑41 (MQ=38)                                   ttGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTc                                                             >  1:4205542/1‑37 (MQ=255)                                   ttGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTa                                                           >  1:4026732/1‑39 (MQ=255)                                   ttGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTAtc                                                         >  1:1028938/1‑41 (MQ=255)                                   ttGTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGtcgtcg                             >  1:4652828/1‑69 (MQ=255)                                     gTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTgg                          <  1:674234/70‑1 (MQ=255)                                     gTCCGCGCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTgg                          <  1:3420959/70‑1 (MQ=255)                                        cgcgCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCAtt                      <  1:1356724/71‑1 (MQ=255)                                        cgcgCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCAtt                      <  1:28483/71‑1 (MQ=255)                                        cgcgCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCAtt                      <  1:1558539/71‑1 (MQ=255)                                        cgcgCCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCAtt                      <  1:1490545/71‑1 (MQ=255)                                         gcgcCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCAtt                      <  1:3829745/70‑1 (MQ=255)                                           gcCACCAACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAAt                   <  1:4467789/71‑1 (MQ=255)                                               ccaACTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCg               <  1:3573850/71‑1 (MQ=255)                                                 aaCTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAAt                   >  1:4270525/1‑65 (MQ=255)                                                 aaCTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTg             >  1:4092624/1‑71 (MQ=255)                                                 aaCTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTACGTg             >  1:806914/1‑71 (MQ=255)                                                  aCTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTgg            <  1:4558439/71‑1 (MQ=255)                                                  aCTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTgg            <  1:249588/71‑1 (MQ=255)                                                   cTCGGTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTgg                          <  1:160587/56‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCTGTGGaa          <  1:6096/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:937869/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:4679825/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:991614/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:4249401/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:4266549/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:805953/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:4369000/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:577932/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:237707/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:1080951/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:1121576/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:1530359/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:1575393/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:1711252/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:1898861/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:2043881/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:2076548/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:2331892/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:2368678/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:3947037/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:2425965/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:2506066/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:2577899/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:3120551/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:3627636/69‑1 (MQ=255)                                                      ggTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:3921249/69‑1 (MQ=255)                                                       gTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCGGTGGaa          <  1:1297001/68‑1 (MQ=255)                                                       gTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:3438225/68‑1 (MQ=255)                                                       gTTATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGaa          <  1:4157756/68‑1 (MQ=255)                                                         tATCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTTTCGTCGTGGCATTAATTCCGTGGAata        >  1:510390/1‑68 (MQ=255)                                                          aTCTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGAATATGc     <  1:3752528/70‑1 (MQ=255)                                                            cTGGCGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTc                 >  1:3542317/1‑56 (MQ=255)                                                                cGTCTTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGAATATGCCCg  <  1:4545280/67‑1 (MQ=255)                                                                   |                                                                GCCGCCGCGGAATCCGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCGGTTATCTGGCGTATTTCTATCTTCTATTTATGTTCTATTTTTGTCGTCGTGGCATTAATTCCGTGGAATATGCCCG  >  W3110S.gb/2793575‑2793704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑