| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,802,461 | G→A | A102T (GCC→ACC) | nrdF → | ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,802,461 | 0 | G | A | 98.7% | 219.9 / ‑4.2 | 74 | A102T (GCC→ACC) | nrdF | ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (30/43); total (31/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
CCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTG > W3110S.gb/2802396‑2802528 | ccGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGc < 1:2616106/71‑1 (MQ=255)ccGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGc < 1:2304077/71‑1 (MQ=255)ccGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGc < 1:3540897/71‑1 (MQ=255) cACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTa > 1:1885642/1‑70 (MQ=255) cACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGTAGCGGTTCATACCCGCTCTTACAGt > 1:2442085/1‑70 (MQ=255) cACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGt > 1:1651071/1‑70 (MQ=255) cATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGAttt > 1:3270638/1‑70 (MQ=255) gaagaaGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtctc > 1:3650990/1‑71 (MQ=255) aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCg > 1:3975042/1‑62 (MQ=255) aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtctc > 1:3926340/1‑70 (MQ=255) aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtctc > 1:2642164/1‑70 (MQ=255) aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtctc > 1:3624655/1‑70 (MQ=255) aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtct > 1:4471758/1‑69 (MQ=255) aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtct > 1:3301730/1‑69 (MQ=255) agCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:460821/71‑1 (MQ=255) agCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:535315/71‑1 (MQ=255) gCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCt < 1:4316478/71‑1 (MQ=255) gCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCt < 1:2477677/71‑1 (MQ=255) tattatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGc < 1:2037606/71‑1 (MQ=255) attatCAAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGc > 1:235004/1‑70 (MQ=255) ttatCGAATATCAGCTTTATGGAAGCGGTTCATAcc > 1:4373577/1‑36 (MQ=255) ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTAc < 1:1868976/45‑1 (MQ=255) ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:1840793/64‑1 (MQ=255) ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:1302365/64‑1 (MQ=255) ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:783020/64‑1 (MQ=255) ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:4629129/64‑1 (MQ=255) ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:1661491/64‑1 (MQ=255) ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGc > 1:3251722/1‑69 (MQ=255) atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGctc > 1:640407/1‑39 (MQ=255) atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGa > 1:3401730/1‑50 (MQ=255) atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:3737678/1‑71 (MQ=255) atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:3030599/1‑71 (MQ=255) atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:2682377/1‑71 (MQ=255) atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:1554441/1‑71 (MQ=255) atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:2075280/1‑71 (MQ=255) atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:2096439/1‑71 (MQ=255) cGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTa > 1:240711/1‑40 (MQ=255) aaTATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:1359116/58‑1 (MQ=255) atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:924798/71‑1 (MQ=255) atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:4559116/71‑1 (MQ=255) atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:1985747/71‑1 (MQ=255) atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:3475734/71‑1 (MQ=255) atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:3439165/71‑1 (MQ=255) atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:214844/71‑1 (MQ=255) atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:2943449/71‑1 (MQ=255) tatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCa > 1:1624593/1‑68 (MQ=255) tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTa > 1:107953/1‑56 (MQ=255) tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGAt < 1:247153/71‑1 (MQ=255) tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGAt > 1:2053559/1‑71 (MQ=255) tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGAt < 1:3101157/71‑1 (MQ=255) tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGAt > 1:3110854/1‑71 (MQ=255) aGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGa > 1:3247521/1‑49 (MQ=255) aGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc > 1:4558681/1‑52 (MQ=255) gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:1713630/65‑1 (MQ=255) gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:1448622/71‑1 (MQ=255) gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:510176/71‑1 (MQ=255) gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:2717497/71‑1 (MQ=255) gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:3017186/71‑1 (MQ=255) gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:2528591/71‑1 (MQ=255) gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:4399195/71‑1 (MQ=255) gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:171461/71‑1 (MQ=255) gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:3908174/71‑1 (MQ=255) cTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:3570337/70‑1 (MQ=255) cTTTATGGAAGCGGTTCATACACGCTCTTACAGTTCGATTTTCTCGACGc < 1:4298971/50‑1 (MQ=255) tttATTGAAGCGGTTCATACCCGCTCTTACAGTTCTATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:1797417/69‑1 (MQ=255) tttATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:424809/49‑1 (MQ=255) tttATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:4196408/69‑1 (MQ=255) aaGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGGCGATgccgcc < 1:3324781/71‑1 (MQ=255) aGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:1404171/61‑1 (MQ=255) ggTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACg > 1:2126107/1‑71 (MQ=255) ttCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:2891648/50‑1 (MQ=255) ttCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGcc < 1:3035371/71‑1 (MQ=255) ttCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGcc < 1:3040780/71‑1 (MQ=255) cATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTg > 1:1747527/1‑71 (MQ=255) | CCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTG > W3110S.gb/2802396‑2802528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |