Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,855,194	A→T	I90N (ATC→AAC)	ygbA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,855,194	0	A	T	100.0%	122.2 / NA	41	I90N (ATC→AAC)	ygbA	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/19);  total (22/19)

GGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTG  >  W3110S.gb/2855128‑2855260                                                                   |                                                                   ggACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCg                                                                 <  1:1233530/70‑1 (MQ=255) ggACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCg                                                                 <  1:3652961/70‑1 (MQ=255)         tttttCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGc                                                         >  1:2833512/1‑70 (MQ=255)         tttttCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGc                                                         >  1:4548128/1‑70 (MQ=255)              ccGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg                                                    <  1:2737053/70‑1 (MQ=255)              ccGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg                                                    <  1:2498559/70‑1 (MQ=255)               cGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGc                                                   <  1:2648757/70‑1 (MQ=255)                ggCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCAt                                                 <  1:4048093/71‑1 (MQ=255)                    gTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCg                                                                 <  1:3216675/51‑1 (MQ=255)                      tCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcgg                                           >  1:2777502/1‑71 (MQ=255)                       cTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggc                                          >  1:1635341/1‑71 (MQ=255)                       cTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggc                                          >  1:4645150/1‑71 (MQ=255)                       cTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggc                                          >  1:2876709/1‑71 (MQ=255)                       cTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggc                                          >  1:3626463/1‑71 (MQ=255)                           cACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg                                                    <  1:3938261/57‑1 (MQ=255)                              cGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggcccggcc                                    >  1:3543739/1‑70 (MQ=255)                              cGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCggcccggccc                                   <  1:4005312/71‑1 (MQ=255)                                gACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCaa                                 >  1:917346/1‑71 (MQ=255)                                gACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCaa                                 >  1:1399636/1‑71 (MQ=255)                                    cTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg                                                    <  1:3314602/48‑1 (MQ=255)                                       gTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCAt                            <  1:611361/69‑1 (MQ=255)                                        tCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATaa                          <  1:584221/70‑1 (MQ=255)                                           tCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg                                                    <  1:695549/41‑1 (MQ=255)                                             gatgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGtt                    >  1:646919/1‑71 (MQ=255)                                             gatgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGtt                    >  1:101475/1‑71 (MQ=255)                                             gatgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGtt                    >  1:1380052/1‑71 (MQ=255)                                             gatgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGt                     <  1:4683993/70‑1 (MQ=255)                                               tgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGttt                   >  1:3038891/1‑70 (MQ=255)                                                gagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCa                 >  1:3286573/1‑71 (MQ=255)                                                     gaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATctct            >  1:3184173/1‑71 (MQ=255)                                                     gaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATctct            >  1:4351534/1‑71 (MQ=255)                                                     gaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATctct            >  1:1183965/1‑71 (MQ=255)                                                     gaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATctct            >  1:1000227/1‑71 (MQ=255)                                                      aCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCtt           <  1:2434697/71‑1 (MQ=255)                                                      aCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCtt           <  1:4015166/71‑1 (MQ=255)                                                      aCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCtt           <  1:1545498/71‑1 (MQ=255)                                                       cGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTTTTTCATCTCtt           >  1:1732914/1‑70 (MQ=255)                                                          aCGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACg       <  1:1198283/71‑1 (MQ=255)                                                           gggTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGc      >  1:2443900/2‑71 (MQ=255)                                                              ttAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCttt   >  1:1577393/1‑71 (MQ=255)                                                                aaGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTg  <  1:1540025/70‑1 (MQ=255)                                                                   |                                                                   GGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTG  >  W3110S.gb/2855128‑2855260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑