Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,867,422 A→G intergenic (‑13/+127) nlpD ← / ← pcm predicted outer membrane lipoprotein/L‑isoaspartate protein carboxylmethyltransferase type II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,867,4220AG100.0% 82.9 / NA 25intergenic (‑13/+127)nlpD/pcmpredicted outer membrane lipoprotein/L‑isoaspartate protein carboxylmethyltransferase type II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/17);  total (8/17)

ACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATG  >  W3110S.gb/2867354‑2867488
                                                                    |                                                                  
aCCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGgaa                                                                  <  1:3697518/71‑1 (MQ=255)
aCCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGgaa                                                                  <  1:2936572/71‑1 (MQ=255)
aCCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGgaa                                                                  <  1:2877775/71‑1 (MQ=255)
               gCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCGAGGGAAAATTGGTTAATAAcc                                                   <  1:151206/71‑1 (MQ=255)
               gCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAAcc                                                   <  1:1424143/71‑1 (MQ=255)
                    gcggcgAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCACCCAGGGAAAATTGGTTAATAACCAGTGa                                              >  1:3961730/1‑71 (MQ=255)
                    gcggcgAACGGTGAATTTTGGGCTTCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGa                                              >  1:1613448/1‑70 (MQ=255)
                     cggcgAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGa                                              >  1:2455234/1‑70 (MQ=255)
                       gcgAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACAt                                           <  1:4275116/71‑1 (MQ=255)
                          aaCGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAAt                                        <  1:2325988/71‑1 (MQ=255)
                            cGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTa                                      >  1:709955/1‑71 (MQ=255)
                                 aaTTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTg                                 >  1:4188784/1‑71 (MQ=255)
                                        ggCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCa                          <  1:1365439/71‑1 (MQ=255)
                                         gCTTCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCAc                         <  1:200393/71‑1 (MQ=255)
                                         gCTTCCAGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCAc                         <  1:89559/71‑1 (MQ=255)
                                            tCCCGCGCTCATTTATCCCCCAGGGAAAATTGGTtaat                                                       <  1:2126754/38‑1 (MQ=255)
                                             cccGCGCTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCt                      <  1:1449343/70‑1 (MQ=255)
                                              ccGCGCTCATTTATCCCCCAGGGAAAATTGGTtaat                                                       <  1:2036106/36‑1 (MQ=255)
                                                   cTCATTTATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCCCCCTACTg                  >  1:3470431/1‑68 (MQ=255)
                                                        ttATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTg           >  1:2760885/1‑70 (MQ=255)
                                                         tATCCCCCAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTgg          >  1:267682/1‑70 (MQ=255)
                                                             ccccAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGaaaa      <  1:3308747/70‑1 (MQ=255)
                                                             ccccAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGaaaa      <  1:1117121/70‑1 (MQ=255)
                                                             ccccAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGaaaa      <  1:1333381/70‑1 (MQ=255)
                                                             ccccAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGaaaa      <  1:4365074/70‑1 (MQ=255)
                                                             ccccAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAg     <  1:553770/71‑1 (MQ=255)
                                                                cAGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATg  <  1:1135561/71‑1 (MQ=255)
                                                                 aGGGAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAg     <  1:1204623/67‑1 (MQ=255)
                                                                    |                                                                  
ACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATG  >  W3110S.gb/2867354‑2867488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: