| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,893,179 | A→C | I110L (ATT→CTT) | ygcP → | predicted anti‑terminator regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,893,179 | 0 | A | C | 98.4% | 211.0 / ‑5.2 | 62 | I110L (ATT→CTT) | ygcP | predicted anti‑terminator regulatory protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (31/30); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
AGTACCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGA > W3110S.gb/2893113‑2893246 | aGTACCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCttgt > 1:218168/1‑71 (MQ=255) tACCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCttgtt > 1:902712/1‑70 (MQ=255) ccAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGAt > 1:2101925/1‑71 (MQ=255) ccAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGAt > 1:2162641/1‑71 (MQ=255) ccAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGAt > 1:2902625/1‑71 (MQ=255) ccAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGAt > 1:447992/1‑71 (MQ=255) ccAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGAt > 1:1622028/1‑71 (MQ=255) ccAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGAt > 1:4608995/1‑71 (MQ=255) aaaGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTc < 1:3929996/71‑1 (MQ=255) aaaGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTc < 1:2590397/71‑1 (MQ=255) aaGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCa < 1:1270504/71‑1 (MQ=255) gCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAAt > 1:3893913/1‑71 (MQ=255) gCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAAt > 1:2671138/1‑71 (MQ=255) gCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAAt > 1:257924/1‑71 (MQ=255) tGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGttt < 1:4210412/71‑1 (MQ=255) tGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGttt > 1:1185900/1‑71 (MQ=255) gTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGttt > 1:3558421/1‑70 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCaa > 1:1321932/1‑61 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAAtt > 1:2998189/1‑63 (MQ=255) ttGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:2444982/1‑71 (MQ=255) tGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTca > 1:2869320/1‑70 (MQ=255) aaaGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaa < 1:1107148/71‑1 (MQ=255) aaGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaac < 1:649923/71‑1 (MQ=255) aGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaaca > 1:1202466/1‑71 (MQ=255) aGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaaca > 1:3638626/1‑71 (MQ=255) aGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaaca > 1:825339/1‑71 (MQ=255) cGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACAtt < 1:832199/71‑1 (MQ=255) cGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACAtt < 1:1929773/71‑1 (MQ=255) cGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACAtt < 1:2598107/71‑1 (MQ=255) cGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACAtt < 1:1099224/71‑1 (MQ=255) cAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCttgttc < 1:940002/45‑2 (MQ=255) cAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaa < 1:1980280/64‑1 (MQ=255) cAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaa < 1:1438530/64‑1 (MQ=255) agCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTc > 1:435460/1‑45 (MQ=255) ggAAGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAg < 1:1588650/71‑1 (MQ=255) aGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTGAATTTCGttt > 1:3680222/1‑49 (MQ=255) aGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGttt > 1:4473031/1‑49 (MQ=255) aGGTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTg > 1:2765768/1‑71 (MQ=255) ggTTTTTTCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTgc > 1:1625672/1‑71 (MQ=255) tttttCTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCacaa > 1:1867482/1‑50 (MQ=255) cTGTATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCg < 1:1829868/70‑1 (MQ=255) tATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTcaca < 1:396735/41‑1 (MQ=255) tATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTcaca < 1:2627017/41‑1 (MQ=255) tATTCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGaa < 1:2130044/69‑1 (MQ=255) ttCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTg < 1:1448616/45‑1 (MQ=255) ttCATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTg < 1:637587/45‑1 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa > 1:2977291/1‑71 (MQ=255) cATCGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa > 1:2518561/1‑71 (MQ=255) cGCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg > 1:2438182/1‑71 (MQ=255) gCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:2978350/70‑1 (MQ=255) gCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:1878601/70‑1 (MQ=255) gCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:1883911/70‑1 (MQ=255) gCCTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg > 1:733391/1‑70 (MQ=255) gCCTGTTTCTTGTT‑ATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTa > 1:1893537/1‑71 (MQ=255) cTGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:821607/68‑1 (MQ=255) tGTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:3543812/67‑1 (MQ=255) gTTTCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTAtt > 1:2209795/1‑70 (MQ=255) tttCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGa < 1:2822559/55‑1 (MQ=255) tttCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCg < 1:2914138/60‑1 (MQ=255) tttCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCg < 1:2276543/60‑1 (MQ=255) tttCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:400369/65‑1 (MQ=255) tttCTTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTga < 1:3213817/71‑1 (MQ=255) | AGTACCAAAGCCTCAATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGA > W3110S.gb/2893113‑2893246 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |