BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,894,340	C→T	W32* (TGG→TGA)	ygcQ ←	predicted flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,894,340	0	C	T	100.0%	178.8 / NA	58	W32* (TGG→TGA)	ygcQ	predicted flavoprotein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (48/10); total (48/10)

CGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTC  >  W3110S.gb/2894275‑2894403

cGCTGCCACTGTTTCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                                            >  1:144263/1‑71 (MQ=255)

cGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTTAGGTTCAATCTGTCAGTg                                                            >  1:4415330/1‑71 (MQ=255)

cGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAg                                                              >  1:4023393/1‑69 (MQ=255)

cGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                                            >  1:3278111/1‑71 (MQ=255)

cGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg                                                            >  1:4618330/1‑71 (MQ=255)

     ccACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGcc                                                        <  1:3639770/70‑1 (MQ=255)

     ccACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGcc                                                        <  1:323633/70‑1 (MQ=255)

          gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAggg                                                    >  1:3717808/1‑69 (MQ=255)

          gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGt                                                   >  1:2087681/1‑70 (MQ=255)

          gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTa                                                  >  1:117786/1‑71 (MQ=255)

          gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTa                                                  >  1:3459900/1‑71 (MQ=255)

          gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTa                                                  >  1:266442/1‑71 (MQ=255)

          gTTCCACCAACGCATCCAGCACCTGTTCAGACACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGt                                                   >  1:1272520/1‑70 (MQ=255)

           ttCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGt                                                   <  1:3602563/69‑1 (MQ=255)

             ccaccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACa                                                <  1:2590924/70‑1 (MQ=255)

                  aaCGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCt                                          >  1:3479621/1‑71 (MQ=255)

                          cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc                                              >  1:3439755/1‑59 (MQ=255)

                          cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCt                                  >  1:1652806/1‑71 (MQ=255)

                          cAGCACCTGTTCCGACACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc                                              >  1:1524413/1‑59 (MQ=255)

                             cACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc                                              >  1:762614/1‑56 (MQ=255)

                               ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTgcgc                              >  1:756701/1‑70 (MQ=255)

                               ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc                             >  1:3562693/1‑71 (MQ=255)

                               ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc                             >  1:3433486/1‑71 (MQ=255)

                               ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc                             >  1:3498493/1‑71 (MQ=255)

                               ccTGTTCCACCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc                             >  1:709480/1‑71 (MQ=255)

                                cTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccg                            >  1:3246460/1‑71 (MQ=255)

                                 tGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc                           >  1:4047409/1‑71 (MQ=255)

                                  gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc                           >  1:3590138/1‑70 (MQ=255)

                                  gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc                           >  1:123578/1‑70 (MQ=255)

                                  gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc                           >  1:1301145/1‑70 (MQ=255)

                                  gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc                           >  1:2924053/1‑70 (MQ=255)

                                  gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc                           >  1:1526456/1‑70 (MQ=255)

                                     ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc                                              >  1:3248674/1‑48 (MQ=255)

                                     ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc                                              >  1:2877263/1‑48 (MQ=255)

                                     ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCgccagc                       >  1:1092643/1‑71 (MQ=255)

                                     ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCgccagc                       >  1:2456754/1‑71 (MQ=255)

                                     ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCgccagc                       >  1:1982810/1‑71 (MQ=255)

                                             aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTg                >  1:1481412/1‑70 (MQ=255)

                                             aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTg                >  1:3797129/1‑70 (MQ=255)

                                             aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTg                >  1:389639/1‑70 (MQ=255)

                                             aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc               >  1:439117/1‑71 (MQ=255)

                                             aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc               >  1:3859271/1‑71 (MQ=255)

                                              cAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc               >  1:3479991/1‑70 (MQ=255)

                                              cAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc               >  1:4274848/1‑70 (MQ=255)

                                              cAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCt              <  1:1153863/71‑1 (MQ=255)

                                                ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTAt            <  1:4556896/71‑1 (MQ=255)

                                                   tGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTg          >  1:3296974/1‑70 (MQ=255)

                                                   tGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGc         >  1:2667706/1‑71 (MQ=255)

                                                       gTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATc                                    >  1:1900918/1‑40 (MQ=255)

                                                       gTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCa     <  1:1960701/71‑1 (MQ=255)

                                                        ttCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCAt    >  1:965644/1‑71 (MQ=255)

                                                          cAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTa             >  1:2954861/1‑60 (MQ=255)

                                                          cAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTa             >  1:2949499/1‑60 (MQ=255)

                                                          cAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATtc  >  1:2767083/1‑71 (MQ=255)

                                                          cAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATtc  >  1:1285201/1‑71 (MQ=255)

                                                           aaTCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCa     <  1:3983099/67‑1 (MQ=255)

                                                            aTCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc               <  1:686959/56‑1 (MQ=255)

                                                            aTCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc               <  1:1711420/56‑1 (MQ=255)

CGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTC  >  W3110S.gb/2894275‑2894403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`