| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,509,602 | Δ1 bp | coding (67/213 nt) | rpmE ← | 50S ribosomal subunit protein L31 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,509,599 | 0 | T | . | 100.0% | 20.1 / NA | 6 | coding (70/213 nt) | rpmE | 50S ribosomal subunit protein L31 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (0/6); total (0/6) | |||||||||||
CAACGGTGGAGCGGATTTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCG > W3110S.gb/3509584‑3509649 | cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:1508064/65‑1 (MQ=255)cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:1991062/65‑1 (MQ=255)cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:2314134/65‑1 (MQ=255)cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:2363969/65‑1 (MQ=255)cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:713925/65‑1 (MQ=255)cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:735875/65‑1 (MQ=255) | CAACGGTGGAGCGGATTTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCG > W3110S.gb/3509584‑3509649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |