Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,371,276	(A)5→3	intergenic (‑9/+296)	dcuA ← / ← aspA	C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,371,273	0	A	.	100.0%	33.6 / NA	9	intergenic (‑6/+300)	dcuA/aspA	C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (0/9);  total (0/9)
*	W3110S.gb	4,371,274	0	A	.	100.0%	33.6 / NA	9	intergenic (‑7/+299)	dcuA/aspA	C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (0/9);  total (0/9)

CGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCA  >  W3110S.gb/4371212‑4371295                                                              ||                      cgcAGCAGCTTTTCTTTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:1624132/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:875238/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:139029/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:1516172/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:161152/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:719610/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:1976725/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:389422/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:2534381/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                     >  1:2276785/1‑64 (MQ=255) cgcAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaa                       >  1:810733/1‑63 (MQ=255)                   tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa  <  1:2004743/64‑1 (MQ=255)                   tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa  <  1:2132297/64‑1 (MQ=255)                   tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa  <  1:1176513/64‑1 (MQ=255)                   tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa  <  1:1991876/64‑1 (MQ=255)                   tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa  <  1:1938074/64‑1 (MQ=255)                   tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa  <  1:1864029/64‑1 (MQ=255)                   tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa  <  1:1265240/64‑1 (MQ=255)                    gatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa  <  1:2356015/63‑1 (MQ=255)                     atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa  <  1:273337/62‑1 (MQ=255)                                                              ||                      CGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCA  >  W3110S.gb/4371212‑4371295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑