Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,395,175	(T)5→4	coding (1017/1053 nt)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,395,171	0	T	.	100.0%	96.2 / NA	23	coding (1021/1053 nt)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/0);  total (23/0)

TTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAA  >  W3110S.gb/4395132‑4395197                                        |                           ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTgc                  >  1:1483509/1‑49 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:2377934/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:994368/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:938239/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:779356/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:646255/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:645196/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:604604/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:553886/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:453746/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:435157/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:2525565/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:2463147/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:1093192/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:2123610/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:1776514/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:1757393/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:1699519/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:1688332/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:161812/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:1580624/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:1547031/1‑65 (MQ=255) ttAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCG‑TTTTACCTGCGCCATGCTTTCCAGaa  >  1:1242185/1‑65 (MQ=255)                                        |                           TTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAA  >  W3110S.gb/4395132‑4395197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑