Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,473,254 Δ1 bp pseudogene (6149/8622 nt)|pseudogene (851/3324 nt) ydbA →
ydbA →
ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein
ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,473,2530G.100.0% 20.1 / NA 6pseudogene (6148/8622 nt)
pseudogene (850/3324 nt)
ydbA
ydbA
ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein
ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (0/6);  total (0/6)

ATACCAGCGGTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCG  >  W3110S.gb/1473245‑1473310
        |                                                         
aTACCAGCGTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg  <  1:1917093/65‑1 (MQ=255)
aTACCAGCGTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg  <  1:2102148/65‑1 (MQ=255)
aTACCAGCGTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg  <  1:347952/65‑1 (MQ=255)
aTACCAGCGTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg  <  1:383562/65‑1 (MQ=255)
aTACCAGCGTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCg  <  1:842151/65‑1 (MQ=255)
 tACCAGCGTGGCATCATCAATAACTTAGTATAACTTGACAAGGGTGCCGTCATTAAAAATGCCg  <  1:1495149/64‑1 (MQ=255)
        |                                                         
ATACCAGCGGTGGCATCATCAATAACTTAGTAAAACTTGACAAGGGTGCCGTCATTAAAAATGCCG  >  W3110S.gb/1473245‑1473310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: