Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,027,474	(T)7→6	intergenic (+124/‑174)	yodD → / → yedP	hypothetical protein/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,027,468	0	T	.	100.0%	282.1 / NA	63	intergenic (+118/‑180)	yodD/yedP	hypothetical protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (30/33);  total (30/33)

TACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGA  >  W3110S.gb/2027428‑2027506                                         |                                       tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:2007543/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:549959/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:171094/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:442659/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1812649/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1860066/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1874227/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1879338/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1934609/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1981744/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:988974/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:2107941/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:257871/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:2442735/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:2438762/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:2205600/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:224661/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:2288825/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:2360770/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:645184/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1271380/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:133579/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1337731/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:85182/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1169067/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:665550/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1502946/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1534507/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:971933/65‑1 (MQ=255) tACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:166076/65‑1 (MQ=255)     ccAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCa           <  1:1581739/65‑1 (MQ=255)     ccAGGAAATCTGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCa           <  1:1761150/65‑1 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:2004243/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:2366740/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:921234/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:592615/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:874164/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:263887/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:832362/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:390934/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:613812/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:51601/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1682726/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1072058/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1102374/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1124439/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1290780/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1373316/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1414622/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1422547/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1539258/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:2275988/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1685387/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1797323/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1860424/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:1001353/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:2122369/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:2133508/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:2201244/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatg   >  1:709187/1‑63 (MQ=255)               tGATGTGTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATaagaatga  >  1:1790434/1‑64 (MQ=255)               tGATGTGTTCATCACCTTATCAGCAA‑TTTTTTCGCTGACAAGAAAATATTCGCCATatgaatga  >  1:2370298/1‑64 (MQ=255)                  tgtgTTCATCACCTTATCCGCAA‑TTTTTTCGCTGACAAGAAAATATTc               <  1:1525050/48‑1 (MQ=39)                                         |                                       TACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGA  >  W3110S.gb/2027428‑2027506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑