Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,649,364	Δ1 bp	coding (1580/4962 nt)	yfhM ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,649,361	0	A	.	100.0%	118.2 / NA	29	coding (1583/4962 nt)	yfhM	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (0/29);  total (0/29)

AATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTA  >  W3110S.gb/2649313‑2649381                                                 |                     aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa     <  1:1180248/65‑1 (MQ=255) aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa     <  1:1851662/65‑1 (MQ=255) aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa     <  1:524063/65‑1 (MQ=255) aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa     <  1:1533608/65‑1 (MQ=255) aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCaaa     <  1:526955/65‑1 (MQ=255)    tCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:2181512/65‑1 (MQ=255)    tCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:849925/65‑1 (MQ=255)    tCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:1336223/65‑1 (MQ=255)     cAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGCAAAGTa  <  1:2039533/64‑1 (MQ=255)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAATTTGCCCTTGc        <  1:767106/44‑1 (MQ=38)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:757415/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:243419/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:2431016/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:2326116/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:2293726/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:921011/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1909083/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:2029235/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1947695/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1004108/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1896633/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1806801/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1774524/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1694466/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1668725/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1524950/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1387570/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGTTGCCCTTGc        <  1:1020755/44‑1 (MQ=39)                   gCTGACACAGCTTCACGCAGTGGGCGCAGG‑AAAGGTGCCCTTGc        <  1:1142368/44‑1 (MQ=38)                                                 |                     AATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTA  >  W3110S.gb/2649313‑2649381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑