Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,938,015	+G	intergenic (+49/‑9)	fucU → / → fucR	L‑fucose mutarotase/DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,938,011	1	.	G	100.0%	70.1 / NA	20	intergenic (+45/‑13)	fucU/fucR	L‑fucose mutarotase/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (20/0);  total (20/0)

TACGCCCGCATGACGCGGGCGGTTATCGAATGAT‑GGGGTGAAAAATATGAAAGCGGCACGCCA  >  W3110S.gb/2937978‑2938040                                   |                              tACGCCCGCATGCCGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCCCGCCa  >  1:1658339/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGa               >  1:1610071/1‑51 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:1909094/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:466849/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:309009/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:304245/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:292490/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:278645/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:2532567/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:2379682/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:2262612/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:2122243/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:102766/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:1821652/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:1787245/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:17793/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:1669791/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:1649923/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:1403750/1‑64 (MQ=255) tACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGTGAAAAATATGAAAGCGGCACGCCa  >  1:1101697/1‑64 (MQ=255)                                   |                              TACGCCCGCATGACGCGGGCGGTTATCGAATGAT‑GGGGTGAAAAATATGAAAGCGGCACGCCA  >  W3110S.gb/2937978‑2938040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑