Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,345,965	+T	coding (1394/1434 nt)	rpoN →	RNA polymerase, sigma 54 (sigma N) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,345,962	1	.	T	100.0%	78.5 / NA	23	S464F (TCT→TTT)	rpoN	RNA polymerase, sigma 54 (sigma N) factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (23/0);  total (23/0)

TGGCACGCCGCACTGTTGCGAAGTACCGAGAGTC‑TTTATCCATTCCGCCGTCAAACCAGCGTA  >  W3110S.gb/3345929‑3345991                                   |                              tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2319545/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:842477/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:809376/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:750366/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:617340/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:509440/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:351009/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:27447/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2487058/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2365751/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2347985/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1181362/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2257782/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2065778/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1976883/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1966866/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1845610/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1837802/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1669241/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1641163/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1564627/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1431473/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1396948/1‑64 (MQ=255) tggCACGCCGCACTGTTGCGAAGTACCGAGAGTC‑t                              >  1:624339/1‑35 (MQ=255)                                   |                              TGGCACGCCGCACTGTTGCGAAGTACCGAGAGTC‑TTTATCCATTCCGCCGTCAAACCAGCGTA  >  W3110S.gb/3345929‑3345991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑