| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,359,804 | +A | coding (752/1419 nt) | gltD → | glutamate synthase, 4Fe‑4S protein, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,359,800 | 1 | . | A | 100.0% | 11.7 / NA | 5 | E250K (GAA→AAA) | gltD | glutamate synthase, 4Fe‑4S protein, small subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/5); total (0/5) | |||||||||||
TGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑AAAACGAAGACGCCGATGG > W3110S.gb/3359756‑3359819 | tgtTCCTTGGCGTCGGGACTTCTCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:2399819/65‑1 (MQ=255)tgtTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:1450777/65‑1 (MQ=255)tgtTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:3254/65‑1 (MQ=255) ccTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:2053182/61‑1 (MQ=255) aCTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:2000095/48‑1 (MQ=39) | TGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑AAAACGAAGACGCCGATGG > W3110S.gb/3359756‑3359819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |