| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,785,326 | +C | coding (1170/1185 nt) | emrD ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,785,322 | 1 | . | C | 100.0% | 20.2 / NA | 7 | Q392E (CAG→GAG) | emrD | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (0/7); total (0/7) | |||||||||||
GCGCTTAAACGGGCTG‑CCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAT > W3110S.gb/3785307‑3785370 | gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:1040820/65‑1 (MQ=255)gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:1105181/65‑1 (MQ=255)gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:1325787/65‑1 (MQ=255)gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:135929/65‑1 (MQ=255)gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:399686/65‑1 (MQ=255)gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:447678/65‑1 (MQ=255)gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:78375/65‑1 (MQ=255) | GCGCTTAAACGGGCTG‑CCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAT > W3110S.gb/3785307‑3785370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |