Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,002,862	(T)7→8	intergenic (‑87/‑145)	pitA ← / → yhiN	phosphate transporter, low‑affinity/predicted oxidoreductase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,002,855	1	.	T	100.0%	110.4 / NA	34	intergenic (‑80/‑152)	pitA/yhiN	phosphate transporter, low‑affinity/predicted oxidoreductase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (5/29);  total (5/29)

GGCGCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGAC‑TTTTTTTGATTTGCCC  >  W3110S.gb/4002808‑4002871                                                 |                 ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:2039802/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:884141/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:793576/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:740235/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:657635/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:652986/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:625761/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:440486/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:389829/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:367498/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:359530/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:338288/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:2323705/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:2298220/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:2023754/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:2015333/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1123588/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1946375/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1938562/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1279216/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1681040/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1503152/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1498997/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1355353/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1306637/65‑1 (MQ=255) ggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATCTGccc  <  1:1841920/65‑1 (MQ=25)  gcgcATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1088700/64‑1 (MQ=255)         aTCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:411629/57‑1 (MQ=37)         aTCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  <  1:1340591/57‑1 (MQ=37)                       cggcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  >  1:1065111/1‑43 (MQ=38)                       cggcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  >  1:31149/1‑43 (MQ=38)                       cggcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  >  1:2160877/1‑43 (MQ=38)                       cggcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  >  1:1053829/1‑43 (MQ=38)                       cggcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc  >  1:1960449/1‑43 (MQ=38)                                                 |                 GGCGCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGAC‑TTTTTTTGATTTGCCC  >  W3110S.gb/4002808‑4002871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑