Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,170,323 (T)5→6 intergenic (+50/‑242) tufA → / → chiA protein chain elongation factor EF‑Tu/periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,170,3181.T87.5% 19.4 / ‑2.5 8intergenic (+45/‑247)tufA/chiAprotein chain elongation factor EF‑Tu/periplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (7/0);  total (7/1)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTGCATTTGTTGACGGCATATCTTATTCGTACTGTCAGAACGAATAAATTA  >  W3110S.gb/4170269‑4170372
                                                  |                                                      
gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCAttt                                             >  1:1766348/1‑62 (MQ=255)
gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt                                          >  1:1362186/1‑65 (MQ=255)
gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt                                          >  1:1577325/1‑65 (MQ=255)
gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt                                          >  1:1750068/1‑65 (MQ=255)
gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt                                          >  1:1799784/1‑65 (MQ=255)
gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt                                          >  1:1844533/1‑65 (MQ=255)
gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt                                          >  1:1926386/1‑65 (MQ=255)
                                        ttCGGCGCCCTTTTTGCATTTGTTGACGGCATATCTTATTCGTACTGTCAGAACGAATAAatta  <  1:1717030/64‑1 (MQ=255)
                                                  |                                                      
GCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTGCATTTGTTGACGGCATATCTTATTCGTACTGTCAGAACGAATAAATTA  >  W3110S.gb/4170269‑4170372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: