Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 4,170,323 | (T)5→6 | intergenic (+50/‑242) | tufA → / → chiA | protein chain elongation factor EF‑Tu/periplasmic endochitinase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 4,170,318 | 1 | . | T | 87.5% | 19.4 / ‑2.5 | 8 | intergenic (+45/‑247) | tufA/chiA | protein chain elongation factor EF‑Tu/periplasmic endochitinase |
Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (7/0); total (7/1) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.25e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCC‑TTTTTGCATTTGTTGACGGCATATCTTATTCGTACTGTCAGAACGAATAAATTA > W3110S.gb/4170269‑4170372 | gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCAttt > 1:1766348/1‑62 (MQ=255) gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt > 1:1362186/1‑65 (MQ=255) gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt > 1:1577325/1‑65 (MQ=255) gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt > 1:1750068/1‑65 (MQ=255) gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt > 1:1799784/1‑65 (MQ=255) gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt > 1:1844533/1‑65 (MQ=255) gCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCCTTTTTTGCATttgtt > 1:1926386/1‑65 (MQ=255) ttCGGCGCCC‑TTTTTGCATTTGTTGACGGCATATCTTATTCGTACTGTCAGAACGAATAAatta < 1:1717030/64‑1 (MQ=255) | GCTAATTACACGTTAATTAGTTTTGAATTGAAAAGGGCGCTTCGGCGCCC‑TTTTTGCATTTGTTGACGGCATATCTTATTCGTACTGTCAGAACGAATAAATTA > W3110S.gb/4170269‑4170372 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |