| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,313,148 | (G)5→6 | coding (11/696 nt) | alsE ← | allulose‑6‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,313,143 | 1 | . | G | 96.3% | 93.7 / ‑2.8 | 27 | coding (16/696 nt) | alsE | allulose‑6‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (26/0); total (27/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑GGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACGGTCAAGGGCGACAGCCGCGAT > W3110S.gb/4313087‑4313207 | gCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:1136845/60‑1 (MQ=255)gCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:313111/60‑1 (MQ=255)gCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:579750/60‑1 (MQ=255)gCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:703072/60‑1 (MQ=255)gCTGTCGATAAATTCGATCTGTTCTTAAAATTTCAGCAGATCCATACACATTAACGA‑ggg < 1:1772728/60‑1 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:752918/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:727832/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:770199/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:686355/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:793908/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:551663/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:537026/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:363265/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:337798/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:859279/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:247631/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:2363782/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1027948/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:2047848/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:2002759/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1798737/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1697290/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:166091/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1637637/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1530248/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:144582/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:130450/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1252973/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1245524/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1208078/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGGAGAtt > 1:1178637/1‑64 (MQ=255) tCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑GGGGGAGAtttt > 1:964319/1‑65 (MQ=255) a‑GGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACGGTCAAGGGCGACAGCCGCGAt > 1:2207125/1‑65 (MQ=255) | GCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGA‑GGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACGGTCAAGGGCGACAGCCGCGAT > W3110S.gb/4313087‑4313207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |