| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,315,771 | (ATC)3→4 | intergenic (‑111/+16) | alsA ← / ← alsB | fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily/D‑allose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,315,761 | 1 | . | C | 93.9% | 115.5 / ‑0.1 | 33 | intergenic (‑101/+26) | alsA/alsB | fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily/D‑allose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (31/0); total (33/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| * | W3110S.gb | 4,315,761 | 2 | . | A | 93.8% | 96.4 / 0.9 | 33 | intergenic (‑101/+26) | alsA/alsB | fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily/D‑allose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (30/0); total (33/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | W3110S.gb | 4,315,761 | 3 | . | T | 93.9% | 108.3 / ‑0.0 | 33 | intergenic (‑101/+26) | alsA/alsB | fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily/D‑allose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (31/0); total (33/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAG‑‑‑CATCATCATCCGGCATCATTC > W3110S.gb/4315701‑4315782 ||| ggCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAG‑‑‑ca < 1:917461/63‑1 (MQ=255)ggCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAG‑‑‑ca < 1:356299/63‑1 (MQ=255)ggCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAG‑‑‑ca < 1:1739890/63‑1 (MQ=255) tACGTTCAGGTTAATTTGTTGGCCGGATAAGACACTTCATTAG‑‑‑ca < 1:1663301/45‑1 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCTTCATCATCATCCGGCATCATTc > 1:542073/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:612427/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:2452841/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:2502439/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:303492/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:310340/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:383026/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:490937/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:609530/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1138826/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:721830/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:806127/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:829656/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:853254/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:968562/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:993562/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1849801/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1074359/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1183877/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1330287/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1401561/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1642711/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1661510/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1765405/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1824037/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:187508/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1911001/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:1940595/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:208255/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:2309175/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCATCCGGCATCATTc > 1:2312193/1‑65 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAG‑‑‑ca > 1:253193/1‑43 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAG‑‑‑Catcatcatca > 1:2311305/1‑51 (MQ=255) cGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAG‑‑‑Catcatcatc > 1:2337952/1‑51 (MQ=255) ||| GGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAG‑‑‑CATCATCATCCGGCATCATTC > W3110S.gb/4315701‑4315782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |