Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	233,963	(GT)4→5	intergenic (‑8/+64)	mltD ← / ← gloB	predicted membrane‑bound lytic murein transglycosylase D/predicted hydroxyacylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	233,954	1	.	T	100.0%	22.9 / NA	8	M1K (ATG→AAG) †	mltD	predicted membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (8/0);  total (8/0)
*	W3110S.gb	233,954	2	.	G	100.0%	24.1 / NA	8	M1T (ATG→ACG) †	mltD	predicted membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (8/0);  total (8/0)

GTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑TGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAT  >  W3110S.gb/233893‑233989                                                               ||                                    gTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑tgt                                  >  1:338469/1‑65 (MQ=255) gTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑tgt                                  >  1:675592/1‑65 (MQ=255)                                    agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAt  >  1:1067369/1‑64 (MQ=255)                                    agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAt  >  1:1137704/1‑64 (MQ=255)                                    agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAt  >  1:1158216/1‑64 (MQ=255)                                    agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAt  >  1:1461778/1‑64 (MQ=255)                                    agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAt  >  1:2036939/1‑64 (MQ=255)                                    agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAt  >  1:394132/1‑64 (MQ=255)                                    agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAt  >  1:887654/1‑64 (MQ=255)                                    agGCGAGTAATATCGCTTTTGCCTTCATGTGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAt  >  1:992029/1‑64 (MQ=255)                                                               ||                                    GTTGCCGGTACTCTGGCAACCCACGAGCAGGACAGAGGCGAGTAATATCGCTTTTGCCTTCA‑‑TGTGTGTGTCAATAGTTGCTTAAAAGACGACCGAT  >  W3110S.gb/233893‑233989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑