Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,478,290	(T)5→6	coding (1433/2307 nt)	ydbD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,478,285	1	.	T	84.6%	33.5 / 1.6	13	coding (1428/2307 nt)	ydbD	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (11/0);  total (11/2)
Fisher's exact test for biased strand distribution p-value = 1.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTA‑TTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAGAAAAAATCCCC  >  W3110S.gb/1478240‑1478347                                               |                                                               tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:1163408/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:1434377/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:1477833/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:1739541/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:2452654/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:2523049/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:252594/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:382608/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:54243/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:70699/1‑65 (MQ=255) tttATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTATTTTTTAGCATTGCCAGAt                                              >  1:761226/1‑65 (MQ=255)                                            ttA‑TTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAGAAAAAATcccg  <  1:682190/65‑2 (MQ=255)                                            ttA‑TTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAGAAAAAATcccc  <  1:1221937/65‑1 (MQ=255)                                               |                                                               TTTATACCAAAAATTCGAGAATGACGATGATCTAATACGTGTGTTA‑TTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAGAAAAAATCCCC  >  W3110S.gb/1478240‑1478347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑