Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,498,266	+T	coding (1265/1344 nt)	ydcJ →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,498,264	1	.	T	100.0%	84.7 / NA	25	A421A (GCG→GCT)	ydcJ	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (25/0);  total (25/0)

CAGTCGCGAAGCATTTGAGCAGGCG‑TTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCA  >  W3110S.gb/1498240‑1498302                          |                                       cAGTCGCGAGGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:55772/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTAcc   >  1:1715666/1‑63 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:2117843/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:983691/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:860188/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:694927/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:680286/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:504191/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:449079/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:414960/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:389639/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:274757/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:2420758/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:213604/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:1015396/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:2035219/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:2008424/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:1574441/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:1469158/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:1339051/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:1275018/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:1222974/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:1202605/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCa  >  1:1166807/1‑64 (MQ=255) cAGTCGCGAAGCATTTGAGCAGGCGTTTGGGTTGTCCGGTTTTGGATGAATTCCAGCTTTACCa  >  1:1289275/1‑64 (MQ=255)                          |                                       CAGTCGCGAAGCATTTGAGCAGGCG‑TTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCA  >  W3110S.gb/1498240‑1498302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑