Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	297,472	+T	coding (479/957 nt)	yagQ ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	297,469	1	.	T	100.0%	18.6 / NA	8	T161N (ACC→AAC)	yagQ	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (0/8);  total (0/8)

GATTCCAGCCCGTCCGGG‑TTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGC  >  W3110S.gb/297452‑297514                   |                                              gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc  <  1:1027161/64‑1 (MQ=255) gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc  <  1:1478402/64‑1 (MQ=255) gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc  <  1:1925432/64‑1 (MQ=255) gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc  <  1:2369678/64‑1 (MQ=255) gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc  <  1:355589/64‑1 (MQ=255) gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc  <  1:431074/64‑1 (MQ=255) gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc  <  1:664171/64‑1 (MQ=255)   ttCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc  <  1:1746960/62‑1 (MQ=255)                   |                                              GATTCCAGCCCGTCCGGG‑TTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGC  >  W3110S.gb/297452‑297514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑