Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,720,897	+C	coding (420/1110 nt)	ydhH ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,720,894	1	.	C	100.0%	72.7 / NA	20	G141G (GGA→GGG)	ydhH	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/0);  total (20/0)

TCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑CCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCC  >  W3110S.gb/1720835‑1720941                                                             |                                                tCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑cccca                                            <  1:528854/65‑3 (MQ=255) tCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑cccca                                            <  1:522280/65‑3 (MQ=255) tCGGTTGGGGGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑cccca                                            <  1:1809458/65‑3 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCAcc                 >  1:2306083/1‑50 (MQ=39)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:1279363/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:622190/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:611976/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:50992/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:2401949/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:2182723/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:2139724/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:2088733/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:2078719/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:204118/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:1966898/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:1839371/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:1784053/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:1305449/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:1303419/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:1076121/1‑65 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTc   >  1:409585/1‑64 (MQ=255)                                            gCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTc   >  1:628045/1‑64 (MQ=255)                                            gCGGTGCGCCTTGTCATCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTcc  >  1:1935232/1‑65 (MQ=255)                                                             |                                                TCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑CCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCC  >  W3110S.gb/1720835‑1720941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑