Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,995,418	(T)5→6	coding (1426/1833 nt)	uvrC ←	excinuclease UvrABC, endonuclease subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,995,413	1	.	T	100.0%	85.4 / NA	27	N477K (AAT→AAA)	uvrC	excinuclease UvrABC, endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (0/27);  total (0/27)

CGAAGACA‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGT  >  W3110S.gb/1995406‑1995477         |                                                                 ctaaGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:2504608/62‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGTGa           <  1:3106/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:228495/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:224747/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:393514/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:2290765/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:510119/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:561997/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:212575/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:2300807/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1904076/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:189652/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1891550/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1837336/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1827094/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1827061/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1684372/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:67591/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1673066/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:2440285/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1475044/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:2440775/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1442316/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:948975/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:1251731/64‑1 (MQ=255) cGAAGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACAACCGGGa           <  1:646417/64‑1 (MQ=255)   aaGACATTTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGa           <  1:297354/62‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:397706/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:414824/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:589992/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:812673/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:820439/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:87638/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:969332/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:2235690/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:2532313/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:2288709/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:1103317/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:2164942/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:2162037/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:2153355/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:1923794/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:1886020/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:1683233/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:1510880/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:1452988/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:1328775/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:125960/65‑1 (MQ=255)        a‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt  <  1:119095/65‑1 (MQ=255)         |                                                                 CGAAGACA‑TTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGT  >  W3110S.gb/1995406‑1995477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑