Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 2,025,196 | +C | coding (167/786 nt) | fliR → | flagellar export pore protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,025,192 | 1 | . | C | 97.6% | 147.7 / ‑3.0 | 41 | coding (163/786 nt) | fliR | flagellar export pore protein |
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (0/40); total (0/41) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
GATGATCACGTTCGCCATTG‑C‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTT > W3110S.gb/2025173‑2025236 | gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:413408/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:209567/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:2129233/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:2162701/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:2444473/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:2543295/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:294915/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:298338/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:339290/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:409720/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1109982/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:437623/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:475323/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:546210/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:552821/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:583019/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:627524/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:738524/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:865575/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:908931/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1487924/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1021156/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1173397/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1231510/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1288437/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1320430/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1354754/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1453813/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1503405/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1529075/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1531192/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1723107/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1848967/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1959660/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1978005/65‑1 (MQ=255) gatgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:2037714/65‑1 (MQ=255) gatgatCACGTTCGCCATTG‑CGCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1339949/65‑1 (MQ=255) atgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:8184/64‑1 (MQ=255) atgatCACGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:2056747/64‑1 (MQ=255) cGTTCGCCATTGCC‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:2064042/57‑1 (MQ=255) tCGCCATTGCC‑CCCAGCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt < 1:1423530/54‑1 (MQ=255) | GATGATCACGTTCGCCATTG‑C‑CCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTT > W3110S.gb/2025173‑2025236 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |