Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,383,756	+C	coding (174/1671 nt)	menD ←	bifunctional 2‑oxoglutarate decarboxylase and SHCHC synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,383,752	1	.	C	100.0%	36.4 / NA	11	H60D (CAT→GAT)	menD	bifunctional 2‑oxoglutarate decarboxylase and SHCHC synthase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (11/0);  total (11/0)

CCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATG‑CCCCAACCCACGCTCATCGAA  >  W3110S.gb/2383710‑2383773                                            |                      ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGc          >  1:2375358/1‑57 (MQ=39) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:1269689/1‑65 (MQ=255) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:1431654/1‑65 (MQ=255) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:1964502/1‑65 (MQ=255) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:20438/1‑65 (MQ=255) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:2096408/1‑65 (MQ=255) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:2187671/1‑65 (MQ=255) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:2241286/1‑65 (MQ=255) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:2377051/1‑65 (MQ=255) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:860280/1‑65 (MQ=255) ccACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCCAACCCACGCTCATCGaa  >  1:861739/1‑65 (MQ=255)                                            |                      CCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATG‑CCCCAACCCACGCTCATCGAA  >  W3110S.gb/2383710‑2383773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑