Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,544,768	+T	coding (262/912 nt)	cysM ←	cysteine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,544,765	1	.	T	83.3%	11.5 / ‑2.1	6	coding (265/912 nt)	cysM	cysteine synthase B
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (5/0);  total (5/1)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAA‑TTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCG  >  W3110S.gb/2544703‑2544814                                                                |                                                  ttCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAA‑t                                                  <  1:731491/64‑1 (MQ=255) ttCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAA‑t                                                  <  1:805033/64‑1 (MQ=255)                           cACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTTGATGCGATAGCCTTTCAGCGCGGc                        >  1:2410649/1‑65 (MQ=255)                           cACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTTCATGCGATAGCCTTTCAGCGCgg                         >  1:1475421/1‑64 (MQ=255)                           cACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTTCATGCGATAGCCTTTCAGCGCgg                         >  1:91874/1‑64 (MQ=255)                           cACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTTCATGCGATAGCCTTTCAGCGCGGc                        >  1:285006/1‑65 (MQ=255)                           cACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTTCATGCGATAGCCTTTCAGCGCGGc                        >  1:99441/1‑65 (MQ=255)                                                 gtCGGGCATCAGCAA‑TTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCg  <  1:1110901/64‑1 (MQ=255)                                                                |                                                  TTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAA‑TTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATGCCG  >  W3110S.gb/2544703‑2544814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑