Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,595,278	+A	coding (116/864 nt)	ypfJ ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,595,274	1	.	A	94.1%	47.2 / ‑2.5	17	coding (120/864 nt)	ypfJ	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base A (16/0);  total (16/1)
Fisher's exact test for biased strand distribution p-value = 5.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGT‑AAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCC  >  W3110S.gb/2595228‑2595321                                                |                                                cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGccc                                      >  1:1114947/1‑59 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:895028/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:1007317/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:867034/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:860295/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:781749/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:333474/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:321082/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:2399041/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:228804/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:2246707/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:2242412/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:2239363/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:1945997/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:1381343/1‑65 (MQ=255) cGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAAATCAGCCCGCCttt                                >  1:1126187/1‑65 (MQ=255)                                 aGCACGACTATCAGT‑AAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGcc  <  1:1847732/62‑1 (MQ=255)                                                |                                                CGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGT‑AAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCC  >  W3110S.gb/2595228‑2595321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑