Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,757,239	(T)6→7	coding (18/957 nt)	yfjH ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,757,233	1	.	T	80.0%	46.8 / 8.9	20	coding (24/957 nt)	yfjH	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base T (0/16);  total (1/19)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GTAAAAATTGTATTTTTGGTACATGGG‑TTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCA  >  W3110S.gb/2757207‑2757283                            |                                                   gTAAAAATTGTATTTTTGGTACATGGG‑TTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCaa               >  1:250410/1‑64 (MQ=255)        ttGTATTTTTGGTACATGGG‑TTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTg       <  1:1850807/65‑1 (MQ=255)         tGTATTTTTGGTACATGGG‑TTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTg       <  1:520155/64‑1 (MQ=255)         tGTATTTTTGGTACATGGG‑TTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTg       <  1:1035823/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:2395413/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:878068/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:667827/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:617253/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:571329/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:408622/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:389280/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:2528760/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:2324782/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:2223613/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:2213671/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:2141940/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:2045718/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:1997840/64‑1 (MQ=255)               tttGGTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:1942705/64‑1 (MQ=255)                  ggTACATGGGTTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCa  <  1:1453769/61‑1 (MQ=255)                            |                                                   GTAAAAATTGTATTTTTGGTACATGGG‑TTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCA  >  W3110S.gb/2757207‑2757283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑