Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,866,850	+T	coding (560/1140 nt)	nlpD ←	predicted outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,866,848	1	.	T	100.0%	183.4 / NA	53	G188R (GGA→AGA)	nlpD	predicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (26/27);  total (26/27)

TACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCG  >  W3110S.gb/2866784‑2866903                                                                  |                                                        tACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:1991156/65‑1 (MQ=255) tACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:1904802/65‑1 (MQ=255) tACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:1848778/65‑1 (MQ=255) tACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:1666610/65‑1 (MQ=255) tACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:1173024/65‑1 (MQ=255) tACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:32056/65‑1 (MQ=255) tACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:1458114/65‑1 (MQ=255) tACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:140364/65‑1 (MQ=255) tACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:628434/65‑1 (MQ=255)  aCGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTcc                                                          <  1:1069104/64‑1 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTa     >  1:1446008/1‑64 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:274429/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:908380/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:2189398/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:2276825/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:1002682/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:2355540/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:849781/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:2425956/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:2507602/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:2516011/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:600003/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:835746/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:794580/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:477455/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:792491/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:534136/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:783127/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:543067/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:1874644/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:1468013/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:1425544/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:1793153/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:1180559/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:1915438/1‑65 (MQ=255)                                                       aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTAc    >  1:1967638/1‑65 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1167490/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1154594/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1197533/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:174736/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:857294/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1313728/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1365141/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1385558/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:140103/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:864327/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1461726/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:444906/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1147672/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:31223/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2013530/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2516417/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1761170/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2506426/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2430197/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2339290/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2331360/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2194148/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2167073/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2082708/65‑1 (MQ=255)                                                         cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:207236/65‑1 (MQ=255)                                                          acaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:1770969/64‑1 (MQ=255)                                                          acaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg  <  1:2247245/64‑1 (MQ=255)                                                                  |                                                        TACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCC‑TTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCG  >  W3110S.gb/2866784‑2866903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑