Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	3685137	3685177	41	14 [0]	[0] 17	ilvG	Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

GCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCA  >  W3110S.gb/3685072‑3685136                                                                 | gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:1130198/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:1290908/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:172480/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:1852844/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:2057901/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:2166097/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:2375171/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:24196/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:249575/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:448947/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:579801/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:610323/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:919833/1‑65 (MQ=255) gCGTCACCGGGATGGTCGTAACGCAAGGCATGTACATCACGCAGCTGCGCGCAGTGTAGCTGCCa  >  1:60324/1‑65 (MQ=255)                                                                 | GCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCA  >  W3110S.gb/3685072‑3685136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑