Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	4136452	4136489	38	16 [0]	[0] 25	[frlD]	[frlD]

ATGAATTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCA  >  W3110S.gb/4136387‑4136451                                                                 | atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:1001848/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:1003831/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:1220227/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:1294318/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:1612468/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:1674705/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:1849247/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:1883648/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:1893178/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:2029969/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:2249371/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:262491/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:333688/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:430368/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:660127/1‑65 (MQ=255) atgaatTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCa  >  1:935470/1‑65 (MQ=255)                                                                 | ATGAATTACTCGGATTATAGGGTCGTTTTCCGGCAAGTAACAGCACGTTGGCGTGAAGATGCTCA  >  W3110S.gb/4136387‑4136451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑