Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	4022452	4022472	21	20 [0]	[0] 28	nikE	nickel transporter subunit

ACCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTTC  >  W3110S.gb/4022473‑4022537 |                                                                 aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTcg           >  1:2437023/1‑56 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCt      >  1:298802/1‑61 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:2391531/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:956610/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:674116/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:651485/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:48337/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:38142/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:319646/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:280315/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:2524413/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:2475926/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:2424510/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:2400672/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:2383638/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:2143934/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:2107862/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:2036958/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:1990837/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:1974350/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:1903673/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:1856126/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:1846101/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:1741174/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTtc  >  1:1732451/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTAtc  >  1:2235865/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGCGCGACGCTTTCGCCGCTTTtc  >  1:19391/1‑65 (MQ=255) aCCGCGCGAGGGTACTTTTCCCGCAGACGCTGCGcc                               >  1:1206962/1‑36 (MQ=255) |                                                                 ACCGCGCGAGGGTACTTTTCCCGCAGCCGCTGCGCCCCAGCAGGGCGACGGTTTCGCCGCTTTTC  >  W3110S.gb/4022473‑4022537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑