| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 415,988 | 0 | G | A | 55.8% | 40.4 / 142.0 | 104 | Y63Y (TAC→TAT) | sbcD | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (27/19); new base A (0/58); total (27/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 104 total aligned reads displayed.
ACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAA > W3110S.gb/415941‑416029 | accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:241280/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1556861/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:146114/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1458563/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1456098/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1408306/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:516249/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1795174/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:418133/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1839294/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1936803/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1305190/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:747799/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1037287/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:817552/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:1144648/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:390697/60‑1 (MQ=255)accaccAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCgg < 1:201422/60‑1 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:409819/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:418073/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1892645/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:43787/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:280664/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:442825/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:451165/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:462542/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:478189/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:256309/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:2527268/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:2005291/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:222304/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:964996/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1535155/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1131162/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1398235/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1375132/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1373356/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1215451/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1368132/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1286687/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1544747/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:1314719/1‑65 (MQ=255) gCCAGTTTGCTGTAAATTGAAAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc > 1:2342982/1‑65 (MQ=255) gTTTGCTGTAAATTGACATCAAAACGGTTGTATAACGTGCgg < 1:1308662/42‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:516057/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:49953/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2408955/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2055652/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2485728/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:940630/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2501254/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:926907/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:909733/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:275237/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:746106/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:292007/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:299617/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:782580/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:588399/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:63631/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:715862/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:399978/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:746220/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1320866/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1712178/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1574274/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1548964/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1405482/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1372014/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1370657/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1361981/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1343387/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:178765/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1312469/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1267253/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1174470/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1119388/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1100140/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1086138/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1031851/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1022962/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2065009/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2304980/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:228814/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2286390/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2175638/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2133838/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2107437/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2084296/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2083103/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2332688/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2055895/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1008393/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1923379/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1879364/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1816199/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:181386/64‑1 (MQ=255) gTAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1810298/64‑1 (MQ=255) tAAATTGACAACAAAACGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:1760818/63‑1 (MQ=255) aCGGTTATATAAAGTACGGGCGTAACTGGGCGGCGAGCCGGTATCGaa < 1:2447479/48‑1 (MQ=255) | ACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAA > W3110S.gb/415941‑416029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |