| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 527,255 | 1 | . | C | 63.6% | 46.3 / 26.5 | 22 | coding (83/711 nt) | ylbH | conserved hypothetical protein, rhs‑like |
| Reads supporting (aligned to +/- strand): ref base . (0/8); new base C (14/0); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.13e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATA > W3110S.gb/527204‑527305 | ttccGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:2252128/4‑64 (MQ=17)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:1075742/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:534430/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:364799/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:2529988/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:2166194/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:2070353/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:1938913/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:1732977/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:1466574/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:1349661/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:114879/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:1134753/1‑64 (MQ=255)ttGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCCGCATCAcc > 1:1015857/1‑64 (MQ=255) gCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATa < 1:1688898/65‑1 (MQ=255) gCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATa < 1:141587/65‑1 (MQ=255) gCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATa < 1:2069357/65‑1 (MQ=255) gCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATa < 1:2124632/65‑1 (MQ=255) gCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATa < 1:1272974/65‑1 (MQ=255) gCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATa < 1:2515287/65‑1 (MQ=255) gCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATa < 1:1092467/65‑1 (MQ=255) gCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATa < 1:798968/65‑1 (MQ=255) | TTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAA‑CCCGCATCACCTGCACCAGCCGTACCGGCTGCCGGGGCAGCAGTATGATA > W3110S.gb/527204‑527305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |