| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 716,431 | 1 | . | T | 61.3% | 58.9 / 43.0 | 31 | coding (349/411 nt) | ybfG | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (12/0); new base T (0/19); total (12/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.09e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGG > W3110S.gb/716367‑716473 | tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:2253790/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:909877/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:89059/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:705621/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:672981/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:495765/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:459028/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:2270631/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:1008311/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:2067878/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:2029416/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:188675/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:1689292/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:1650122/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:1513151/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:1069638/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:1099780/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:1256009/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:1349117/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAc > 1:1427477/1‑65 (MQ=255)tCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGATCAGTAAGCGAAATAAc > 1:1786707/1‑65 (MQ=255) cGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTttcttg > 1:569763/1‑65 (MQ=255) cGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTttcttg > 1:1414510/1‑65 (MQ=255) cGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTttcttg > 1:736915/1‑65 (MQ=255) cGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTttcttg > 1:1830248/1‑65 (MQ=255) cGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTttctt > 1:172350/1‑64 (MQ=255) cGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTttctt > 1:1298087/1‑64 (MQ=255) cGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTttctt > 1:1323593/1‑64 (MQ=255) cGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTttctt > 1:264322/1‑64 (MQ=255) cGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTttctt > 1:72640/1‑64 (MQ=255) tctttcATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTTTCTTGTTCTTGcttc > 1:1379810/1‑65 (MQ=255) tctttcATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTTTCTTGTTCTTGcttc > 1:1306599/1‑65 (MQ=255) tctttcATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTTTCTTGTTCTTGcttc > 1:1136320/1‑65 (MQ=255) cgagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:2325212/64‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:70393/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:731827/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:286936/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:861668/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:863317/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:907839/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:1569333/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:630078/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:2512469/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:2415895/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:2415133/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:2413671/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:2315765/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:2300982/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:1813524/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:2137632/65‑1 (MQ=255) agagTTCAGTAAGCGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:2019496/65‑1 (MQ=255) gTAAGGGAAATAACTTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAgg < 1:1931550/57‑1 (MQ=255) | TCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAAC‑TTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGG > W3110S.gb/716367‑716473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |