| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 793,203 | 1 | . | T | 60.0% | 15.0 / 10.9 | 10 | coding (1008/1473 nt) | modF | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base . (4/0); new base T (6/0); total (10/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGCAAACTACTGCTGACGTAACCGATATGC‑TTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAA > W3110S.gb/793173‑793251 | aTGCAAACTACTGCTGACGTAACCGATATGCTTTTTTGATATCCCAGATGGTTTCGCCGCTggc > 1:2493450/1‑62 (MQ=39)aTGCAAACTACTGCTGACGTAACCGATATGCTTTTTTGATATCCCAGATGGTTTCGCCGCTGcc > 1:10028/1‑64 (MQ=255)aTGCAAACTACTGCTGACGTAACCGATATGCTTTTTTGATATCCCAGATGGTTTCGCCGCTGcc > 1:1261140/1‑64 (MQ=255)aTGCAAACTACTGCTGACGTAACCGATATGCTTTTTTGATATCCCAGATGGTTTCGCCGCTGcc > 1:216093/1‑64 (MQ=255)aTGCAAACTACTGCTGACGTAACCGATATGCTTTTTTGATATCCCAGATGGTTTCGCCGCTGcc > 1:608831/1‑64 (MQ=255)aTGCAAACTACTGCTGACGTAAACGATATGCTTTTTTGATATCCCAGATgg > 1:2507180/1‑51 (MQ=255) tgACGTAACCGATATGC‑TTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGaaa > 1:1208045/1‑65 (MQ=255) tgACGTAACCGATATGC‑TTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGaaa > 1:1707084/1‑65 (MQ=255) tgACGTAACCGATATGC‑TTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGaaa > 1:313110/1‑65 (MQ=255) tgACGTAACCGATATGC‑TTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGaa > 1:229327/1‑64 (MQ=255) | ATGCAAACTACTGCTGACGTAACCGATATGC‑TTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAA > W3110S.gb/793173‑793251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |