| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,654,507 | 1 | . | T | 60.0% | 56.8 / 43.4 | 30 | pseudogene (46/111 nt) | ynfP | ECK1574:JW5257:b4534; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (2/10); new base T (18/0); total (20/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAATCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTG > W3110S.gb/1654461‑1654548 | aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTa > 1:1118367/1‑64 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTa > 1:1380398/1‑64 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTa > 1:2220109/1‑64 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTa > 1:1598275/1‑64 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:999835/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:983572/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:666121/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:528344/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:451438/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:327954/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:2211039/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:2079227/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:1151361/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:1757547/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:1213570/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:178083/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGCTTTTCACCAAATCCTTAg > 1:1263174/1‑65 (MQ=255)aaaTCCATAAATTCAAGCGCAGTGCCCAGCCATCCAGATACTGCTGCTTTTCACCAAATCCTTa > 1:1874171/1‑64 (MQ=255) aaTTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTc > 1:1954461/1‑65 (MQ=255) aaTTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTc > 1:2265242/1‑65 (MQ=255) gCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTg < 1:1363723/65‑1 (MQ=255) gCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTg < 1:635803/65‑1 (MQ=255) gCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTg < 1:562389/65‑1 (MQ=255) gCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTg < 1:1315664/65‑1 (MQ=255) gCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTg < 1:253089/65‑1 (MQ=255) gCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTg < 1:248535/65‑1 (MQ=255) gCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTg < 1:1771441/65‑1 (MQ=255) gCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCATTCGTGTTTTTCTATTg < 1:1489582/65‑1 (MQ=255) cccAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTg < 1:1090513/64‑1 (MQ=255) cGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTg < 1:357381/53‑1 (MQ=255) | AAATCCATAAATTCAAGCGCAGTGCCCAGCCATCCCGATACTGCTGC‑TTTCACCAAATCCTTAGTGCTTCTTTCGTGTTTTTCTATTG > W3110S.gb/1654461‑1654548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |