| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,875,261 | 1 | . | T | 56.5% | 134.1 / 117.2 | 69 | intergenic (+16/‑27) | yeaJ/yeaK | predicted diguanylate cyclase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (30/0); new base T (39/0); total (69/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGAA > W3110S.gb/1875212‑1875287 | tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:370671/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1946912/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1967560/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1988604/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:2047142/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:2148764/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:2149202/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:2150067/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:2156021/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:2398775/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:2452453/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:342427/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:348660/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:2295688/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:377107/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:474647/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:524412/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:53429/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:546236/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:68540/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:70294/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:740986/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:811396/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:827152/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:913517/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:988181/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1030042/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1198549/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1195561/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1743196/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1265500/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1183863/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1148382/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:132085/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1095970/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1444310/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1055054/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGTAAt > 1:1325369/1‑63 (MQ=255)tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTTGCTTGAAAt > 1:1051931/1‑63 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGgttg‑t > 1:1223829/1‑39 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGgttg > 1:309269/1‑38 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGgttg > 1:804560/1‑38 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:701128/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:114024/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:611624/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1110179/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:811522/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:859172/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:533287/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:903318/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:914404/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:126445/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1094186/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:2315945/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1732293/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:198490/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1674658/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1999198/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1468009/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1364968/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1357633/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1021165/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:2296935/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:1249824/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:2455227/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:2462375/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:256349/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:310656/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:314864/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:321706/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:361031/1‑64 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGGGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGaa > 1:129771/1‑64 (MQ=255) | TGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTG‑TTTGCTTGTAATCTCAGGAGCGTGAA > W3110S.gb/1875212‑1875287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |