Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,710,046	1	.	T	60.0%	26.0 / 19.1	15	coding (971/1623 nt)	nadB	quinolinate synthase, L‑aspartate oxidase (B protein) subunit
Reads supporting (aligned to +/- strand):  ref base . (6/0);  new base T (9/0);  total (15/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCCGATTTTATTCGCCAGCA‑TTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAT  >  W3110S.gb/2710026‑2710089                      |                                            cgccgATTTTATTCGCCAGCATTTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGa   >  1:528773/1‑64 (MQ=255) cgccgATTTTATTCGCCAGCATTTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:1414575/1‑65 (MQ=255) cgccgATTTTATTCGCCAGCATTTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:1708065/1‑65 (MQ=255) cgccgATTTTATTCGCCAGCATTTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:1743741/1‑65 (MQ=255) cgccgATTTTATTCGCCAGCATTTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:1942004/1‑65 (MQ=255) cgccgATTTTATTCGCCAGCATTTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:1975648/1‑65 (MQ=255) cgccgATTTTATTCGCCAGCATTTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:2243646/1‑65 (MQ=255) cgccgATTTTATTCGCCAGCATTTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:475828/1‑65 (MQ=255) cgccgATTTTATTCGCCAGCATTTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:917902/1‑65 (MQ=255) cgccgATTTTATTCGCCAGCA‑TTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:1447760/1‑64 (MQ=255) cgccgATTTTATTCGCCAGCA‑TTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:1450800/1‑64 (MQ=255) cgccgATTTTATTCGCCAGCA‑TTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:1651455/1‑64 (MQ=255) cgccgATTTTATTCGCCAGCA‑TTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:1749233/1‑64 (MQ=255) cgccgATTTTATTCGCCAGCA‑TTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:2337165/1‑64 (MQ=255) cgccgATTTTATTCGCCAGCA‑TTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAt  >  1:2340493/1‑64 (MQ=255)                      |                                            CGCCGATTTTATTCGCCAGCA‑TTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAT  >  W3110S.gb/2710026‑2710089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑