Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,797,5481.C71.0% 99.7 / 34.2 38intergenic (‑397/‑272)stpA/ygaWDNA binding protein, nucleoid‑associated/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (11/0);  new base C (27/0);  total (38/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAATT  >  W3110S.gb/2797515‑2797595
                                  |                                               
acacCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGa                            >  1:2141206/1‑55 (MQ=255)
acacCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAg                    >  1:672038/1‑63 (MQ=255)
acacCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAg                    >  1:661098/1‑63 (MQ=255)
acacCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAg                    >  1:2140752/1‑63 (MQ=255)
acacCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAg                    >  1:339666/1‑63 (MQ=255)
              aCGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCat          >  1:222417/1‑59 (MQ=255)
              aCGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCat          >  1:133751/1‑59 (MQ=255)
              aCGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCat          >  1:375220/1‑59 (MQ=255)
              aCGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCat          >  1:367982/1‑59 (MQ=255)
              aCGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCat          >  1:1748428/1‑59 (MQ=255)
              aCGCTAATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCat          >  1:1350127/1‑59 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:360121/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:2236774/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:2278141/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:229111/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:282304/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:340585/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:767673/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:442843/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:550513/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:751549/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1077269/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:2216814/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:2199075/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:2067036/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:2065135/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1920046/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1908854/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:17714/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1712135/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:162155/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1614745/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1596039/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1577109/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1523282/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1473142/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1338769/1‑65 (MQ=255)
                 cTTATAGCGTTACCTCACCCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAAtt  >  1:1138089/1‑65 (MQ=255)
                                  |                                               
ACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGATATTAAAGGTAAGGCCATATCTAATT  >  W3110S.gb/2797515‑2797595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: