| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,917,088 | 0 | T | C | 58.3% | 53.7 / 120.3 | 96 | T318T (ACA→ACG) | gudD | (D)‑glucarate dehydratase 1 |
| Reads supporting (aligned to +/- strand): ref base T (8/32); new base C (0/56); total (8/88) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAG > W3110S.gb/2917066‑2917138 | ccACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:2084587/1‑65 (MQ=255)ccACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:2406019/1‑65 (MQ=255)ccACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:1249112/1‑65 (MQ=255)ccACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:1238720/1‑65 (MQ=255)ccACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:2229059/1‑65 (MQ=255)ccACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:1734844/1‑65 (MQ=255)ccACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:1091119/1‑65 (MQ=255)ccACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:1526897/1‑65 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAggggg < 1:247037/65‑3 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1689323/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1874173/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:2490847/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1670548/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:2231666/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:2305738/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:231920/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1629126/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:2429931/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1050037/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:249747/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:38900/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:411715/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:434274/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:471831/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:565307/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:671558/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:857223/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:898890/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:927929/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1387377/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:122489/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1446514/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:120328/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1138669/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1124255/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1328366/65‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATAGCAACGGATTGCAGGgag < 1:1993852/65‑1 (MQ=255) gTACCGAACCTTGCATCGTCC‑‑AAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1338539/65‑1 (MQ=255) gTACCGAACCTTGAATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:1056087/65‑1 (MQ=255) tACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:794973/64‑1 (MQ=255) tACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGgag < 1:865686/64‑1 (MQ=255) aacGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1538491/63‑1 (MQ=255) aCCGAACCTTTCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1293032/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAATTGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1501046/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:295053/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:379806/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:377675/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:374645/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:294353/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:290673/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:471021/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:286957/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:279667/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:120844/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:459976/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:470018/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1386205/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:471748/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:485946/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:540943/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1197738/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:653519/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:659880/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:863370/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:890743/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:101204/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1476654/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1605888/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1607476/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1609289/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:161675/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:162167/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1643046/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:151403/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:150726/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:178018/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:181390/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:183397/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1851968/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1368379/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1874593/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:19195/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:210076/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:2110270/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:2113871/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:221081/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1410473/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1405878/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:2348966/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1586485/65‑1 (MQ=255) aCCGAACCTTGCATCGTCCACAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:2252765/65‑1 (MQ=255) aCCGAACCTTGCATCCTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1810236/65‑1 (MQ=255) ccGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:759802/64‑1 (MQ=255) ccGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:143895/64‑1 (MQ=255) ccGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:1412900/64‑1 (MQ=255) gCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGagag < 1:221587/55‑1 (MQ=255) | CCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAG > W3110S.gb/2917066‑2917138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |